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Gene expression analysis by means of RT-qPCR is a highly sensitive technique. However, this requires an accurate protocol for the whole procedure from sampling up to data analysis. We have optimized this protocol specifically for the analysis of plant tissues. Special attention is paid to RNA quality and integrity and to the appropriate setup of the assays in order to be compliant with the MIQE guidelines. This protocol was already successfully applied in ten different plant species.
Original languageEnglish
Title of host publicationQuantitative Real-Time PCR : Methods and protocols
EditorsRoberto Biassoni, Alessandro Raso
Number of pages14
Volume2
Place of PublicationNew York
PublisherHumana Press, Springer
Publication date2020
Pages105-118
ISBN (Print)978-1-4939-9832-6
ISBN (Electronic)978-1-4939-9833-3
Publication statusPublished - 2020

ID: 7558145