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Branching gene expression during chrysanthemum axillary bud outgrowth regulated by strigolactone and auxin transport. / Dierck, Robrecht; Leus, Leen; Dhooghe, Emmy; Van Huylenbroeck, Johan; De Riek, Jan; Van Der Straeten, Dominique; De Keyser, Ellen.

In: Plant Growth Regulation, No. doi.org/10.1007/s1072 5-018-0408-2), doi.org/10.1007/s1072 5-018-0408-2, 05.06.2018, p. 23.

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Dierck R, Leus L, Dhooghe E, Van Huylenbroeck J, De Riek J, Van Der Straeten D et al. Branching gene expression during chrysanthemum axillary bud outgrowth regulated by strigolactone and auxin transport. Plant Growth Regulation. 2018 Jun 5;(doi.org/10.1007/s1072 5-018-0408-2)):23. doi.org/10.1007/s1072 5-018-0408-2. https://doi.org/10.1007/s10725-018-0408-2

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@article{18a3b5135fb047b2b19573d6f08704c7,
title = "Branching gene expression during chrysanthemum axillary bud outgrowth regulated by strigolactone and auxin transport",
abstract = "Shoot branching is essential in ornamental chrysanthemum production and determines final plant shape and quality. Auxinis associated with apical dominance to indirectly inhibit bud outgrowth. Two non-mutually exclusive models exist for indirectauxin inhibition. Basipetal auxin transport inhibits axillary bud outgrowth by limiting auxin export from buds to stem(canalization model) or by increasing strigolactone levels (second messenger model). Here we analyzed bud outgrowth intreatments with auxin (IAA), strigolactone (GR24) and auxin transport inhibitor (NPA) using a split-plate bioassay withisolated chrysanthemum stem segments. Besides measuring bud length, dividing cell percentage was measured with flowcytometry and RT-qPCR was used to monitor expression levels of genes involved in auxin transport (CmPIN1) and signaling(CmAXR2), bud dormancy (CmBRC1, CmDRM1) and strigolactone biosynthesis (CmMAX1, CmMAX3). Treatments over a5-day period showed bud outgrowth in the control and inhibition with IAA and IAA + GR24. Bud outgrowth in the controlcoincided with high dividing cell percentage, decreased expression of CmBRC1 and CmDRM1 and increased CmPIN1 expression.Inhibition by IAA and IAA + GR24 coincided with low dividing cell percentage and unchanged or increased expressionsof CmBRC1, CmDRM1 and CmPIN1. Treatment with GR24 showed restricted bud outgrowth that was counteracted byNPA. This restricted bud outgrowth was still concomitant with a high dividing cell percentage and coincided with decreasedexpression of dormancy genes. These results indicate incomplete inhibition of bud outgrowth by GR24 treatment and suggestinvolvement of auxin transport in the mechanism of bud inhibition by strigolactones, supporting the canalization model.",
author = "Robrecht Dierck and Leen Leus and Emmy Dhooghe and {Van Huylenbroeck}, Johan and {De Riek}, Jan and {Van Der Straeten}, Dominique and {De Keyser}, Ellen",
year = "2018",
month = "6",
day = "5",
doi = "10.1007/s10725-018-0408-2",
language = "English",
pages = "23",
journal = "Plant Growth Regulation",
issn = "0167-6903",
publisher = "Springer Netherlands",
number = "doi.org/10.1007/s1072 5-018-0408-2)",

}

RIS

TY - JOUR

T1 - Branching gene expression during chrysanthemum axillary bud outgrowth regulated by strigolactone and auxin transport

AU - Dierck, Robrecht

AU - Leus, Leen

AU - Dhooghe, Emmy

AU - Van Huylenbroeck, Johan

AU - De Riek, Jan

AU - Van Der Straeten, Dominique

AU - De Keyser, Ellen

PY - 2018/6/5

Y1 - 2018/6/5

N2 - Shoot branching is essential in ornamental chrysanthemum production and determines final plant shape and quality. Auxinis associated with apical dominance to indirectly inhibit bud outgrowth. Two non-mutually exclusive models exist for indirectauxin inhibition. Basipetal auxin transport inhibits axillary bud outgrowth by limiting auxin export from buds to stem(canalization model) or by increasing strigolactone levels (second messenger model). Here we analyzed bud outgrowth intreatments with auxin (IAA), strigolactone (GR24) and auxin transport inhibitor (NPA) using a split-plate bioassay withisolated chrysanthemum stem segments. Besides measuring bud length, dividing cell percentage was measured with flowcytometry and RT-qPCR was used to monitor expression levels of genes involved in auxin transport (CmPIN1) and signaling(CmAXR2), bud dormancy (CmBRC1, CmDRM1) and strigolactone biosynthesis (CmMAX1, CmMAX3). Treatments over a5-day period showed bud outgrowth in the control and inhibition with IAA and IAA + GR24. Bud outgrowth in the controlcoincided with high dividing cell percentage, decreased expression of CmBRC1 and CmDRM1 and increased CmPIN1 expression.Inhibition by IAA and IAA + GR24 coincided with low dividing cell percentage and unchanged or increased expressionsof CmBRC1, CmDRM1 and CmPIN1. Treatment with GR24 showed restricted bud outgrowth that was counteracted byNPA. This restricted bud outgrowth was still concomitant with a high dividing cell percentage and coincided with decreasedexpression of dormancy genes. These results indicate incomplete inhibition of bud outgrowth by GR24 treatment and suggestinvolvement of auxin transport in the mechanism of bud inhibition by strigolactones, supporting the canalization model.

AB - Shoot branching is essential in ornamental chrysanthemum production and determines final plant shape and quality. Auxinis associated with apical dominance to indirectly inhibit bud outgrowth. Two non-mutually exclusive models exist for indirectauxin inhibition. Basipetal auxin transport inhibits axillary bud outgrowth by limiting auxin export from buds to stem(canalization model) or by increasing strigolactone levels (second messenger model). Here we analyzed bud outgrowth intreatments with auxin (IAA), strigolactone (GR24) and auxin transport inhibitor (NPA) using a split-plate bioassay withisolated chrysanthemum stem segments. Besides measuring bud length, dividing cell percentage was measured with flowcytometry and RT-qPCR was used to monitor expression levels of genes involved in auxin transport (CmPIN1) and signaling(CmAXR2), bud dormancy (CmBRC1, CmDRM1) and strigolactone biosynthesis (CmMAX1, CmMAX3). Treatments over a5-day period showed bud outgrowth in the control and inhibition with IAA and IAA + GR24. Bud outgrowth in the controlcoincided with high dividing cell percentage, decreased expression of CmBRC1 and CmDRM1 and increased CmPIN1 expression.Inhibition by IAA and IAA + GR24 coincided with low dividing cell percentage and unchanged or increased expressionsof CmBRC1, CmDRM1 and CmPIN1. Treatment with GR24 showed restricted bud outgrowth that was counteracted byNPA. This restricted bud outgrowth was still concomitant with a high dividing cell percentage and coincided with decreasedexpression of dormancy genes. These results indicate incomplete inhibition of bud outgrowth by GR24 treatment and suggestinvolvement of auxin transport in the mechanism of bud inhibition by strigolactones, supporting the canalization model.

U2 - 10.1007/s10725-018-0408-2

DO - 10.1007/s10725-018-0408-2

M3 - A1: Web of Science-article

SP - 23

JO - Plant Growth Regulation

JF - Plant Growth Regulation

SN - 0167-6903

IS - doi.org/10.1007/s1072 5-018-0408-2)

M1 - doi.org/10.1007/s1072 5-018-0408-2

ER -