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A method for the detection of ethinylestradiol in cattle urine is described, based on enzymic hydrolysis of the sample, clean-up by means of disposable octadecyl and amino solid-phase extraction columns, fractionation by reversed-phase high-performance liquid chromatography, and detection by gas chromatography-mass spectrometry (selected-ion monitoring). Identification is based on both gas chromatographic and mass spectrometric data. The method has been tested on urine samples for a collaborative study and all the results found were correct.
Original languageEnglish
JournalJournal of Chromatography A
Volume564
Issue number2
Pages (from-to)469-75
Number of pages7
ISSN0021-9673
Publication statusPublished - 1991

    Research areas

  • Animals, Cattle, Chromatography, High Pressure Liquid, Ethinyl Estradiol, Gas Chromatography-Mass Spectrometry, Substance Abuse Detection

ID: 280401