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Flow cytometric cell cycle analysis allows for rapid screening of estrogenicity in MCF-7 breast cancer cells. / Vanparys, C; Maras, M; Lenjou, M; Robbens, J; Van Bockstaele, D; Blust, R; De Coen, W.

In: Toxicology in Vitro, Vol. 20, No. 7, 2006, p. 1238-48.

Research output: Contribution to journalA1: Web of Science-article

Harvard

Vanparys, C, Maras, M, Lenjou, M, Robbens, J, Van Bockstaele, D, Blust, R & De Coen, W 2006, 'Flow cytometric cell cycle analysis allows for rapid screening of estrogenicity in MCF-7 breast cancer cells', Toxicology in Vitro, vol. 20, no. 7, pp. 1238-48. https://doi.org/10.1016/j.tiv.2006.05.002

APA

Vanparys, C., Maras, M., Lenjou, M., Robbens, J., Van Bockstaele, D., Blust, R., & De Coen, W. (2006). Flow cytometric cell cycle analysis allows for rapid screening of estrogenicity in MCF-7 breast cancer cells. Toxicology in Vitro, 20(7), 1238-48. https://doi.org/10.1016/j.tiv.2006.05.002

Vancouver

Vanparys C, Maras M, Lenjou M, Robbens J, Van Bockstaele D, Blust R et al. Flow cytometric cell cycle analysis allows for rapid screening of estrogenicity in MCF-7 breast cancer cells. Toxicology in Vitro. 2006;20(7):1238-48. https://doi.org/10.1016/j.tiv.2006.05.002

Author

Vanparys, C ; Maras, M ; Lenjou, M ; Robbens, J ; Van Bockstaele, D ; Blust, R ; De Coen, W. / Flow cytometric cell cycle analysis allows for rapid screening of estrogenicity in MCF-7 breast cancer cells. In: Toxicology in Vitro. 2006 ; Vol. 20, No. 7. pp. 1238-48.

Bibtex

@article{c98d197b88ac4226ae539d009226b13f,
title = "Flow cytometric cell cycle analysis allows for rapid screening of estrogenicity in MCF-7 breast cancer cells",
abstract = "The quantitative measurement of individual cells and their characteristics by means of flow cytometry is already for many years of great value for clinical studies. However, its potential as a tool in (eco)toxicology has only recently been discovered. Analysis of cell cycle kinetics with DNA-staining dyes can offer a valuable alternative to detect effects of chemicals on cell proliferation, an important endpoint in screening estrogen-like properties of chemicals. In the present study, flow cytometric cell cycle analysis in growth arrested MCF-7 cells exposed to five xenoestrogens correspond well with cell proliferation results of the conventionally used E-screen assay. Moreover, re-induction of proliferation in MCF-7 cells, indicated by the percentage of cells in S(ynthesis)-phase, is most pronounced after 24 h exposure, thus allowing a faster screening of xenoestrogens. This flow cytometric proliferation assay confirms that the estrogenic activity of structurally analogous parabens is mediated by the estrogen receptor pathway and is proportional to the alkyl chain length. Moreover, the ER-mediated mode of action of two fluorotelomer alcohols (6:2 FTOH and 8:2 FTOH), recently reported as xenoestrogenic, could be elucidated. These results support the potential of flow cytometric cell cycle kinetics as a screening assay for estrogen-like properties of chemicals.",
keywords = "Alcohols, Benzo(a)pyrene, Cell Line, Tumor, Cell Nucleus Division, Cell Proliferation, DDT, Dose-Response Relationship, Drug, Drug Evaluation, Preclinical, Endosulfan, Estradiol, Estrogens, Flow Cytometry, Fluorocarbons, G0 Phase, Gene Expression, Humans, Parabens, Phenols, Receptors, Estrogen, Reverse Transcriptase Polymerase Chain Reaction, S Phase, Tumor Suppressor Proteins",
author = "C Vanparys and M Maras and M Lenjou and J Robbens and {Van Bockstaele}, D and R Blust and {De Coen}, W",
year = "2006",
doi = "10.1016/j.tiv.2006.05.002",
language = "English",
volume = "20",
pages = "1238--48",
journal = "Toxicology in Vitro",
issn = "1879-3177",
publisher = "Elsevier Limited",
number = "7",

}

RIS

TY - JOUR

T1 - Flow cytometric cell cycle analysis allows for rapid screening of estrogenicity in MCF-7 breast cancer cells

AU - Vanparys, C

AU - Maras, M

AU - Lenjou, M

AU - Robbens, J

AU - Van Bockstaele, D

AU - Blust, R

AU - De Coen, W

PY - 2006

Y1 - 2006

N2 - The quantitative measurement of individual cells and their characteristics by means of flow cytometry is already for many years of great value for clinical studies. However, its potential as a tool in (eco)toxicology has only recently been discovered. Analysis of cell cycle kinetics with DNA-staining dyes can offer a valuable alternative to detect effects of chemicals on cell proliferation, an important endpoint in screening estrogen-like properties of chemicals. In the present study, flow cytometric cell cycle analysis in growth arrested MCF-7 cells exposed to five xenoestrogens correspond well with cell proliferation results of the conventionally used E-screen assay. Moreover, re-induction of proliferation in MCF-7 cells, indicated by the percentage of cells in S(ynthesis)-phase, is most pronounced after 24 h exposure, thus allowing a faster screening of xenoestrogens. This flow cytometric proliferation assay confirms that the estrogenic activity of structurally analogous parabens is mediated by the estrogen receptor pathway and is proportional to the alkyl chain length. Moreover, the ER-mediated mode of action of two fluorotelomer alcohols (6:2 FTOH and 8:2 FTOH), recently reported as xenoestrogenic, could be elucidated. These results support the potential of flow cytometric cell cycle kinetics as a screening assay for estrogen-like properties of chemicals.

AB - The quantitative measurement of individual cells and their characteristics by means of flow cytometry is already for many years of great value for clinical studies. However, its potential as a tool in (eco)toxicology has only recently been discovered. Analysis of cell cycle kinetics with DNA-staining dyes can offer a valuable alternative to detect effects of chemicals on cell proliferation, an important endpoint in screening estrogen-like properties of chemicals. In the present study, flow cytometric cell cycle analysis in growth arrested MCF-7 cells exposed to five xenoestrogens correspond well with cell proliferation results of the conventionally used E-screen assay. Moreover, re-induction of proliferation in MCF-7 cells, indicated by the percentage of cells in S(ynthesis)-phase, is most pronounced after 24 h exposure, thus allowing a faster screening of xenoestrogens. This flow cytometric proliferation assay confirms that the estrogenic activity of structurally analogous parabens is mediated by the estrogen receptor pathway and is proportional to the alkyl chain length. Moreover, the ER-mediated mode of action of two fluorotelomer alcohols (6:2 FTOH and 8:2 FTOH), recently reported as xenoestrogenic, could be elucidated. These results support the potential of flow cytometric cell cycle kinetics as a screening assay for estrogen-like properties of chemicals.

KW - Alcohols

KW - Benzo(a)pyrene

KW - Cell Line, Tumor

KW - Cell Nucleus Division

KW - Cell Proliferation

KW - DDT

KW - Dose-Response Relationship, Drug

KW - Drug Evaluation, Preclinical

KW - Endosulfan

KW - Estradiol

KW - Estrogens

KW - Flow Cytometry

KW - Fluorocarbons

KW - G0 Phase

KW - Gene Expression

KW - Humans

KW - Parabens

KW - Phenols

KW - Receptors, Estrogen

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - S Phase

KW - Tumor Suppressor Proteins

U2 - 10.1016/j.tiv.2006.05.002

DO - 10.1016/j.tiv.2006.05.002

M3 - A1: Web of Science-article

C2 - 16797915

VL - 20

SP - 1238

EP - 1248

JO - Toxicology in Vitro

JF - Toxicology in Vitro

SN - 1879-3177

IS - 7

ER -