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The illegal use of anabolic steroids in livestock breeding has taken enormous proportions the last few decades. To protect the consumer against possible harmful effects due to the consumption of contaminated meat or meat products, a multiresidue analysis of anabolic steroids has been developed for muscle tissues and urine. The pretreatment of the meat and urine samples consists of an enzymatic digestion, liquid or solid-phase extraction, and finally high-performance liquid chromatography (HPLC) fractionation. Five fractions or windows are collected, each containing a number of analytes. The residues are derivatized prior to the detection by gas chromatography-mass spectrometry (GC-MS). Both gas chromatographic retention data and mass spectral data are used for identification of nortestosterone, testosterone, estradiol, ethynylestradiol, trenbolone, zeranol, diethylstilbestrol, boldenone, methandienone, methyltestosterone, megestrol acetate, chlormadinone acetate, medroxyprogesterone acetate, chlorotestosterone, progesterone, and chlorotestosterone acetate. The limit of detection varies from matrix to matrix and from analyte to analyte but is, in the most favorable case, on the order of 0.3 ppb (micrograms/kg).
Original languageEnglish
JournalJournal of Chromatographic Science
Issue number10
Pages (from-to)409-14
Number of pages6
Publication statusPublished - 1992

    Research areas

  • Anabolic Agents, Animals, Cattle, Chromatography, High Pressure Liquid, Drug Residues, Gas Chromatography-Mass Spectrometry, Hydrolysis, Indicators and Reagents, Meat, Muscles, Spectrophotometry, Ultraviolet

ID: 280383