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New and fast detection of Little cherry virus 1 using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP). / Tahzima, Rachid; Foucart, Yoika; Peusens, Gertie; Beliën, Tim; Massart, Sebastien; De Jonghe, Kris.

New and fast detection of Little cherry virus 1 using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP). 2018. p. 126-126.

Research output: Chapter in Book/Report/Conference proceedingC3: Conference Abstract

Harvard

Tahzima, R, Foucart, Y, Peusens, G, Beliën, T, Massart, S & De Jonghe, K 2018, New and fast detection of Little cherry virus 1 using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP). in New and fast detection of Little cherry virus 1 using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP). pp. 126-126, 61st International Symposium on Crop Protection (2009), Gent, Belgium, 22/05/18.

APA

Tahzima, R., Foucart, Y., Peusens, G., Beliën, T., Massart, S., & De Jonghe, K. (2018). New and fast detection of Little cherry virus 1 using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP). In New and fast detection of Little cherry virus 1 using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) (pp. 126-126)

Vancouver

Tahzima R, Foucart Y, Peusens G, Beliën T, Massart S, De Jonghe K. New and fast detection of Little cherry virus 1 using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP). In New and fast detection of Little cherry virus 1 using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP). 2018. p. 126-126

Author

Tahzima, Rachid ; Foucart, Yoika ; Peusens, Gertie ; Beliën, Tim ; Massart, Sebastien ; De Jonghe, Kris. / New and fast detection of Little cherry virus 1 using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP). New and fast detection of Little cherry virus 1 using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP). 2018. pp. 126-126

Bibtex

@inbook{0834d0a9e8074e7b86c560c98e02b1d9,
title = "New and fast detection of Little cherry virus 1 using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP)",
abstract = "Little cherry virus 1 (LChV-1, Velarivirus, Closteroviridae) is an economically important pathogen affect-ing mainly cherry around the world emphasizing the impetus for its efficient and accurate on-site de-tection. This study describes the development of a reliable diagnostic protocol of LChV-1 based on a fast, sensitive, and easy-to-use one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP). The protocol detects all LChV-1 isolates in less than 10 min by fluorescence monitoring using a mobile detection device (GENIE, OptiGene) and is most optimal when incubation is performed at 67 °C. Sharp melting curves and unique melting temperatures (Tm) were obtained for the positive samples. Both the LAMP and classical RT-PCR methods are capable of specifically detecting LChV-1 in infected leaf tissues, but this validated leaf-to-result assay has remarkable advantages in comparison to RT-PCR. It is at least hundred fold more sensitive, significantly faster, and efficient at minimal cost, and is ready for on-field applications. In conclusion, this innovative LAMP approach can contribute to implementation of sustainable integrated management strategies for on-site detection of LChV-1 in commercial orchards or for horticultural research stations. It is also suitable for decision support in phytosanitary epidemiological programs.",
author = "Rachid Tahzima and Yoika Foucart and Gertie Peusens and Tim Beli{\"e}n and Sebastien Massart and {De Jonghe}, Kris",
year = "2018",
month = "5",
day = "22",
language = "English",
pages = "126--126",
booktitle = "New and fast detection of Little cherry virus 1 using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP)",

}

RIS

TY - CHAP

T1 - New and fast detection of Little cherry virus 1 using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP)

AU - Tahzima, Rachid

AU - Foucart, Yoika

AU - Peusens, Gertie

AU - Beliën, Tim

AU - Massart, Sebastien

AU - De Jonghe, Kris

PY - 2018/5/22

Y1 - 2018/5/22

N2 - Little cherry virus 1 (LChV-1, Velarivirus, Closteroviridae) is an economically important pathogen affect-ing mainly cherry around the world emphasizing the impetus for its efficient and accurate on-site de-tection. This study describes the development of a reliable diagnostic protocol of LChV-1 based on a fast, sensitive, and easy-to-use one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP). The protocol detects all LChV-1 isolates in less than 10 min by fluorescence monitoring using a mobile detection device (GENIE, OptiGene) and is most optimal when incubation is performed at 67 °C. Sharp melting curves and unique melting temperatures (Tm) were obtained for the positive samples. Both the LAMP and classical RT-PCR methods are capable of specifically detecting LChV-1 in infected leaf tissues, but this validated leaf-to-result assay has remarkable advantages in comparison to RT-PCR. It is at least hundred fold more sensitive, significantly faster, and efficient at minimal cost, and is ready for on-field applications. In conclusion, this innovative LAMP approach can contribute to implementation of sustainable integrated management strategies for on-site detection of LChV-1 in commercial orchards or for horticultural research stations. It is also suitable for decision support in phytosanitary epidemiological programs.

AB - Little cherry virus 1 (LChV-1, Velarivirus, Closteroviridae) is an economically important pathogen affect-ing mainly cherry around the world emphasizing the impetus for its efficient and accurate on-site de-tection. This study describes the development of a reliable diagnostic protocol of LChV-1 based on a fast, sensitive, and easy-to-use one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP). The protocol detects all LChV-1 isolates in less than 10 min by fluorescence monitoring using a mobile detection device (GENIE, OptiGene) and is most optimal when incubation is performed at 67 °C. Sharp melting curves and unique melting temperatures (Tm) were obtained for the positive samples. Both the LAMP and classical RT-PCR methods are capable of specifically detecting LChV-1 in infected leaf tissues, but this validated leaf-to-result assay has remarkable advantages in comparison to RT-PCR. It is at least hundred fold more sensitive, significantly faster, and efficient at minimal cost, and is ready for on-field applications. In conclusion, this innovative LAMP approach can contribute to implementation of sustainable integrated management strategies for on-site detection of LChV-1 in commercial orchards or for horticultural research stations. It is also suitable for decision support in phytosanitary epidemiological programs.

M3 - C3: Conference Abstract

SP - 126

EP - 126

BT - New and fast detection of Little cherry virus 1 using one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP)

ER -