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Podosphaera pannosa (syn. Sphaerotheca pannosa) on Rosa and Prunus spp.: Characterization of pathotypes by differential plant reactions and ITS sequences. / Leus, L; Dewitte, A; Van Huylenbroeck, J; Vanhoutte, N; Van Bockstaele, E; Hofte, M.

In: Journal of Phytopathology, Vol. 154, No. 1, 2006, p. 23-28.

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Leus, L ; Dewitte, A ; Van Huylenbroeck, J ; Vanhoutte, N ; Van Bockstaele, E ; Hofte, M. / Podosphaera pannosa (syn. Sphaerotheca pannosa) on Rosa and Prunus spp.: Characterization of pathotypes by differential plant reactions and ITS sequences. In: Journal of Phytopathology. 2006 ; Vol. 154, No. 1. pp. 23-28.

Bibtex

@article{2ddde42904bc4ad79c06d9c6f10dd460,
title = "Podosphaera pannosa (syn. Sphaerotheca pannosa) on Rosa and Prunus spp.: Characterization of pathotypes by differential plant reactions and ITS sequences",
abstract = "The powdery mildew fungus Podosphaera pannosa (Wallr.: Fr.) de Bary (syn. Sphaerotheca pannosa) is a major problem on roses worldwide. Twenty-six monoconidial isolates of Podosphaera collected on roses and Prunus spp. in Belgium, Germany, France, Denmark, Israel and The Netherlands were characterized on the basis of differential reactions on in vitro rose genotypes and Prunus avium L. and by DNA sequence analysis of the rDNA ITS (internal transcribed spacer) region. Twenty-four isolates were determined as P. pannosa. Amongst these, different groups could be distinguished. A first group of 18 isolates was highly virulent on rose and avirulent or very weakly virulent on P. avium. A second group of four isolates was highly virulent on both rose and P. avium. Analysis of the ITS sequence could discriminate these two groups of P. pannosa strains by a one base pair difference. Finally, two isolates of powdery mildew collected on Prunus sp. could be classified as P. pannosa based on their ITS sequence, which was identical to the ITS sequence of the isolates only highly virulent on roses. However, these two isolates were not able to infect roses. These results indicate that different strains of P. pannosa exist with varying host specificity. We demonstrated by ITS sequencing and plant reactions that the host range of P. pannosa comprises roses and Prunus spp.",
author = "L Leus and A Dewitte and {Van Huylenbroeck}, J and N Vanhoutte and {Van Bockstaele}, E and M Hofte",
year = "2006",
doi = "10.1111/j.1439-0434.2005.01053.x",
language = "English",
volume = "154",
pages = "23--28",
journal = "Journal of Phytopathology",
issn = "0931-1785",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Podosphaera pannosa (syn. Sphaerotheca pannosa) on Rosa and Prunus spp.: Characterization of pathotypes by differential plant reactions and ITS sequences

AU - Leus, L

AU - Dewitte, A

AU - Van Huylenbroeck, J

AU - Vanhoutte, N

AU - Van Bockstaele, E

AU - Hofte, M

PY - 2006

Y1 - 2006

N2 - The powdery mildew fungus Podosphaera pannosa (Wallr.: Fr.) de Bary (syn. Sphaerotheca pannosa) is a major problem on roses worldwide. Twenty-six monoconidial isolates of Podosphaera collected on roses and Prunus spp. in Belgium, Germany, France, Denmark, Israel and The Netherlands were characterized on the basis of differential reactions on in vitro rose genotypes and Prunus avium L. and by DNA sequence analysis of the rDNA ITS (internal transcribed spacer) region. Twenty-four isolates were determined as P. pannosa. Amongst these, different groups could be distinguished. A first group of 18 isolates was highly virulent on rose and avirulent or very weakly virulent on P. avium. A second group of four isolates was highly virulent on both rose and P. avium. Analysis of the ITS sequence could discriminate these two groups of P. pannosa strains by a one base pair difference. Finally, two isolates of powdery mildew collected on Prunus sp. could be classified as P. pannosa based on their ITS sequence, which was identical to the ITS sequence of the isolates only highly virulent on roses. However, these two isolates were not able to infect roses. These results indicate that different strains of P. pannosa exist with varying host specificity. We demonstrated by ITS sequencing and plant reactions that the host range of P. pannosa comprises roses and Prunus spp.

AB - The powdery mildew fungus Podosphaera pannosa (Wallr.: Fr.) de Bary (syn. Sphaerotheca pannosa) is a major problem on roses worldwide. Twenty-six monoconidial isolates of Podosphaera collected on roses and Prunus spp. in Belgium, Germany, France, Denmark, Israel and The Netherlands were characterized on the basis of differential reactions on in vitro rose genotypes and Prunus avium L. and by DNA sequence analysis of the rDNA ITS (internal transcribed spacer) region. Twenty-four isolates were determined as P. pannosa. Amongst these, different groups could be distinguished. A first group of 18 isolates was highly virulent on rose and avirulent or very weakly virulent on P. avium. A second group of four isolates was highly virulent on both rose and P. avium. Analysis of the ITS sequence could discriminate these two groups of P. pannosa strains by a one base pair difference. Finally, two isolates of powdery mildew collected on Prunus sp. could be classified as P. pannosa based on their ITS sequence, which was identical to the ITS sequence of the isolates only highly virulent on roses. However, these two isolates were not able to infect roses. These results indicate that different strains of P. pannosa exist with varying host specificity. We demonstrated by ITS sequencing and plant reactions that the host range of P. pannosa comprises roses and Prunus spp.

U2 - 10.1111/j.1439-0434.2005.01053.x

DO - 10.1111/j.1439-0434.2005.01053.x

M3 - A1: Web of Science-article

VL - 154

SP - 23

EP - 28

JO - Journal of Phytopathology

JF - Journal of Phytopathology

SN - 0931-1785

IS - 1

ER -