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Nicarbazin and halofuginone have been widely used as coccidiostats for the prevention and treatment of coccidiosis in poultry. It has been shown that accidental cross-contamination of feed can lead to residues of these compounds in eggs and/or muscle. This paper describes a direct competitive assay for detecting halofuginone and nicarbazin, developed as qualitative screening assay. In an optimized competitive ELISA, antibodies showed 50% binding inhibition at approximately 0.08 ng ml(-1) for halofuginone and 2.5 ng ml(-1) for dinitrocarbanilide (marker residue for nicarbazin). Extraction from the matrix was carried out with acetonitrile followed by a wash with hexane. The assay's detection capability (CCbeta) for halofuginone was <0.5 microg kg(-1) in egg and <1 microg kg(-1) in muscle. For dinitrocarbanilide, the CCbeta was estimated at <3 microg kg(-1) in egg and <10 microg kg(-1) in chicken muscle.
Original languageEnglish
JournalFood Additives and Contaminants
Issue number2
Pages (from-to)128-134
Number of pages7
Publication statusPublished - 2005

    Research areas

  • Animals, Antibodies, Carbanilides, Chickens, Coccidiostats, Cross Reactions, Drug Residues, Eggs, Enzyme-Linked Immunosorbent Assay, Food Contamination, Muscles, Nicarbazin, Piperidines, Quinazolines, Quinazolinones, Reproducibility of Results

ID: 280317