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Gene expression analysis by means of RT-qPCR is a highly sensitive technique. However, this requires an accurate protocol for the whole procedure from sampling up to data analysis. We have optimized this protocol specifically for the analysis of plant tissues. Special attention is paid to RNA quality and integrity and to the appropriate setup of the assays in order to be compliant with the MIQE guidelines. This protocol was already successfully applied in ten different plant species.
Originele taal-2Engels
TitelQuantitative Real-Time PCR : Methods and protocols
EditorsRoberto Biassoni, Alessandro Raso
Aantal pagina's14
Volume2
Plaats productieNew York
UitgeverijHumana Press, Springer
Publicatiedatum2020
Pagina's105-118
ISBN van geprinte versie978-1-4939-9832-6
ISBN van elektronische versie978-1-4939-9833-3
StatusGepubliceerd - 2020

ID: 7558145