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A general protocol for accurate gene exprression analysis in plants : qPCR in plants. / De Keyser, Ellen; Desmet, Laurence; Losschaert, Magali; De Riek, Jan.

Quantitative Real-Time PCR: Methods and protocols. ed. / Roberto Biassoni; Alessandro Raso. Vol. 2 New York : Humana Press, Springer, 2020. blz. 105-118 (Methods in Molecular Biology; Vol. 2).

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Harvard

De Keyser, E, Desmet, L, Losschaert, M & De Riek, J 2020, A general protocol for accurate gene exprression analysis in plants: qPCR in plants. in R Biassoni & A Raso (eds), Quantitative Real-Time PCR: Methods and protocols. vol. 2, Methods in Molecular Biology, vol. 2, Humana Press, Springer, New York, blz. 105-118.

APA

De Keyser, E., Desmet, L., Losschaert, M., & De Riek, J. (2020). A general protocol for accurate gene exprression analysis in plants: qPCR in plants. In R. Biassoni, & A. Raso (editors), Quantitative Real-Time PCR: Methods and protocols (Vol. 2, blz. 105-118). (Methods in Molecular Biology; Vol. 2). New York: Humana Press, Springer.

Vancouver

De Keyser E, Desmet L, Losschaert M, De Riek J. A general protocol for accurate gene exprression analysis in plants: qPCR in plants. In Biassoni R, Raso A, editors, Quantitative Real-Time PCR: Methods and protocols. Vol. 2. New York: Humana Press, Springer. 2020. blz. 105-118. (Methods in Molecular Biology).

Author

De Keyser, Ellen ; Desmet, Laurence ; Losschaert, Magali ; De Riek, Jan. / A general protocol for accurate gene exprression analysis in plants : qPCR in plants. Quantitative Real-Time PCR: Methods and protocols. editor / Roberto Biassoni ; Alessandro Raso. Vol. 2 New York : Humana Press, Springer, 2020. blz. 105-118 (Methods in Molecular Biology).

Bibtex

@inbook{3ccbb4d04cae4a3c80ae472939a4cf80,
title = "A general protocol for accurate gene exprression analysis in plants: qPCR in plants",
abstract = "Gene expression analysis by means of RT-qPCR is a highly sensitive technique. However, this requires an accurate protocol for the whole procedure from sampling up to data analysis. We have optimized this protocol specifically for the analysis of plant tissues. Special attention is paid to RNA quality and integrity and to the appropriate setup of the assays in order to be compliant with the MIQE guidelines. This protocol was already successfully applied in ten different plant species.",
author = "{De Keyser}, Ellen and Laurence Desmet and Magali Losschaert and {De Riek}, Jan",
year = "2020",
language = "English",
isbn = "978-1-4939-9832-6",
volume = "2",
series = "Methods in Molecular Biology",
publisher = "Humana Press, Springer",
pages = "105--118",
editor = "Roberto Biassoni and Alessandro Raso",
booktitle = "Quantitative Real-Time PCR",
address = "United States",

}

RIS

TY - CHAP

T1 - A general protocol for accurate gene exprression analysis in plants

T2 - qPCR in plants

AU - De Keyser, Ellen

AU - Desmet, Laurence

AU - Losschaert, Magali

AU - De Riek, Jan

PY - 2020

Y1 - 2020

N2 - Gene expression analysis by means of RT-qPCR is a highly sensitive technique. However, this requires an accurate protocol for the whole procedure from sampling up to data analysis. We have optimized this protocol specifically for the analysis of plant tissues. Special attention is paid to RNA quality and integrity and to the appropriate setup of the assays in order to be compliant with the MIQE guidelines. This protocol was already successfully applied in ten different plant species.

AB - Gene expression analysis by means of RT-qPCR is a highly sensitive technique. However, this requires an accurate protocol for the whole procedure from sampling up to data analysis. We have optimized this protocol specifically for the analysis of plant tissues. Special attention is paid to RNA quality and integrity and to the appropriate setup of the assays in order to be compliant with the MIQE guidelines. This protocol was already successfully applied in ten different plant species.

M3 - Contribution to book/anthology

SN - 978-1-4939-9832-6

VL - 2

T3 - Methods in Molecular Biology

SP - 105

EP - 118

BT - Quantitative Real-Time PCR

A2 - Biassoni, Roberto

A2 - Raso, Alessandro

PB - Humana Press, Springer

CY - New York

ER -