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In vitro inoculation methods are an efficient tool for the accurate study of the interaction between plants and parasitic nematodes. Obtaining disinfected nematodes is a crucial step in most of these methods. Most disinfection protocols involve surface sterilization of hatched juveniles, a procedure that usually leads to high mortality. This paper describes a new protocol for fast, inexpensive and easy disinfection of nematodes of the genus Globodera, directly from cysts. Mortality is low and varies between 10 and 40 % at the most. The surface-sterilized juveniles successfully infected in vitro-grown potato roots and reproduced on them.
Originele taal-2Engels
TijdschriftFundamental And Applied Nematology
Volume19
Nummer van het tijdschrift1
Pagina's (van-tot)91-93
Aantal pagina's3
ISSN1164-5571
StatusGepubliceerd - 1996

ID: 450496