Standard

Bacillus cereus NVH 0500/00 can adhere to mucin but cannot produce enterotoxins during gastrointestinal simulation. / Tsilia, Varvara; Kerckhof, Frederiek-Maarten; Rajkovic, Andreja; Heyndrickx, Marc; Van de Wiele, Tom.

In: Applied and environmental microbiology, Vol. 82, Nr. 1, 2016, blz. 289-96.

Onderzoeksoutput: Bijdrage aan tijdschriftA2: Artikel in een internationaal wetenschappelijk tijdschrift met peer review, dat niet inbegrepen is in A1

Harvard

Tsilia, V, Kerckhof, F-M, Rajkovic, A, Heyndrickx, M & Van de Wiele, T 2016, 'Bacillus cereus NVH 0500/00 can adhere to mucin but cannot produce enterotoxins during gastrointestinal simulation', Applied and environmental microbiology, vol. 82, nr. 1, blz. 289-96. https://doi.org/10.1128/AEM.02940-15

APA

Tsilia, V., Kerckhof, F-M., Rajkovic, A., Heyndrickx, M., & Van de Wiele, T. (2016). Bacillus cereus NVH 0500/00 can adhere to mucin but cannot produce enterotoxins during gastrointestinal simulation. Applied and environmental microbiology, 82(1), 289-96. https://doi.org/10.1128/AEM.02940-15

Vancouver

Tsilia V, Kerckhof F-M, Rajkovic A, Heyndrickx M, Van de Wiele T. Bacillus cereus NVH 0500/00 can adhere to mucin but cannot produce enterotoxins during gastrointestinal simulation. Applied and environmental microbiology. 2016;82(1):289-96. https://doi.org/10.1128/AEM.02940-15

Author

Tsilia, Varvara ; Kerckhof, Frederiek-Maarten ; Rajkovic, Andreja ; Heyndrickx, Marc ; Van de Wiele, Tom. / Bacillus cereus NVH 0500/00 can adhere to mucin but cannot produce enterotoxins during gastrointestinal simulation. In: Applied and environmental microbiology. 2016 ; Vol. 82, Nr. 1. blz. 289-96.

Bibtex

@article{49d6fcfb5c9c4684bd922b4145b80818,
title = "Bacillus cereus NVH 0500/00 can adhere to mucin but cannot produce enterotoxins during gastrointestinal simulation",
abstract = "Adhesion to the intestinal epithelium could constitute an essential mechanism of Bacillus cereus pathogenesis. However, the enterocytes are protected by mucus, a secretion composed mainly of mucin glycoproteins. These may serve as nutrients and sites of adhesion for intestinal bacteria. In this study, the food poisoning bacterium B. cereus NVH 0500/00 was exposed in vitro to gastrointestinal hurdles prior to evaluation of its attachment to mucin microcosms and its ability to produce nonhemolytic enterotoxin (Nhe). The persistence of mucin-adherent B. cereus after simulated gut emptying was determined using a mucin adhesion assay. The stability of Nhe toward bile and pancreatin (intestinal components) in the presence of mucin agar was also investigated. B. cereus could grow and simultaneously adhere to mucin during in vitro ileal incubation, despite the adverse effect of prior exposure to a low pH or intestinal components. The final concentration of B. cereus in the simulated lumen at 8 h of incubation was 6.62 ± 0.87 log CFU ml(-1). At that point, the percentage of adhesion was approximately 6{\%}. No enterotoxin was detected in the ileum, due to either insufficient bacterial concentrations or Nhe degradation. Nevertheless, mucin appears to retain B. cereus and to supply it to the small intestine after simulated gut emptying. Additionally, mucin may play a role in the protection of enterotoxins from degradation by intestinal components.",
author = "Varvara Tsilia and Frederiek-Maarten Kerckhof and Andreja Rajkovic and Marc Heyndrickx and {Van de Wiele}, Tom",
note = "Copyright {\circledC} 2015, American Society for Microbiology. All Rights Reserved.",
year = "2016",
doi = "10.1128/AEM.02940-15",
language = "English",
volume = "82",
pages = "289--96",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "Amer Soc Microbiology",
number = "1",

}

RIS

TY - JOUR

T1 - Bacillus cereus NVH 0500/00 can adhere to mucin but cannot produce enterotoxins during gastrointestinal simulation

AU - Tsilia, Varvara

AU - Kerckhof, Frederiek-Maarten

AU - Rajkovic, Andreja

AU - Heyndrickx, Marc

AU - Van de Wiele, Tom

N1 - Copyright © 2015, American Society for Microbiology. All Rights Reserved.

PY - 2016

Y1 - 2016

N2 - Adhesion to the intestinal epithelium could constitute an essential mechanism of Bacillus cereus pathogenesis. However, the enterocytes are protected by mucus, a secretion composed mainly of mucin glycoproteins. These may serve as nutrients and sites of adhesion for intestinal bacteria. In this study, the food poisoning bacterium B. cereus NVH 0500/00 was exposed in vitro to gastrointestinal hurdles prior to evaluation of its attachment to mucin microcosms and its ability to produce nonhemolytic enterotoxin (Nhe). The persistence of mucin-adherent B. cereus after simulated gut emptying was determined using a mucin adhesion assay. The stability of Nhe toward bile and pancreatin (intestinal components) in the presence of mucin agar was also investigated. B. cereus could grow and simultaneously adhere to mucin during in vitro ileal incubation, despite the adverse effect of prior exposure to a low pH or intestinal components. The final concentration of B. cereus in the simulated lumen at 8 h of incubation was 6.62 ± 0.87 log CFU ml(-1). At that point, the percentage of adhesion was approximately 6%. No enterotoxin was detected in the ileum, due to either insufficient bacterial concentrations or Nhe degradation. Nevertheless, mucin appears to retain B. cereus and to supply it to the small intestine after simulated gut emptying. Additionally, mucin may play a role in the protection of enterotoxins from degradation by intestinal components.

AB - Adhesion to the intestinal epithelium could constitute an essential mechanism of Bacillus cereus pathogenesis. However, the enterocytes are protected by mucus, a secretion composed mainly of mucin glycoproteins. These may serve as nutrients and sites of adhesion for intestinal bacteria. In this study, the food poisoning bacterium B. cereus NVH 0500/00 was exposed in vitro to gastrointestinal hurdles prior to evaluation of its attachment to mucin microcosms and its ability to produce nonhemolytic enterotoxin (Nhe). The persistence of mucin-adherent B. cereus after simulated gut emptying was determined using a mucin adhesion assay. The stability of Nhe toward bile and pancreatin (intestinal components) in the presence of mucin agar was also investigated. B. cereus could grow and simultaneously adhere to mucin during in vitro ileal incubation, despite the adverse effect of prior exposure to a low pH or intestinal components. The final concentration of B. cereus in the simulated lumen at 8 h of incubation was 6.62 ± 0.87 log CFU ml(-1). At that point, the percentage of adhesion was approximately 6%. No enterotoxin was detected in the ileum, due to either insufficient bacterial concentrations or Nhe degradation. Nevertheless, mucin appears to retain B. cereus and to supply it to the small intestine after simulated gut emptying. Additionally, mucin may play a role in the protection of enterotoxins from degradation by intestinal components.

U2 - 10.1128/AEM.02940-15

DO - 10.1128/AEM.02940-15

M3 - A2: International peer reviewed article (not A1-type)

C2 - 26497468

VL - 82

SP - 289

EP - 296

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 1

ER -