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Biofilm models for the food industry : hot spots for plasmid transfer? / Van Meervenne, Eva; De Weirdt, Rosemarie; Van Coillie, Els; Devlieghere, Frank; Herman, Lieve; Boon, Nico.

In: Pathogens and Disease, Vol. 70, Nr. 3, 2014, blz. 332-8.

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikelOnderzoekpeer review

Harvard

Van Meervenne, E, De Weirdt, R, Van Coillie, E, Devlieghere, F, Herman, L & Boon, N 2014, 'Biofilm models for the food industry: hot spots for plasmid transfer?' Pathogens and Disease, vol. 70, nr. 3, blz. 332-8. https://doi.org/10.1111/2049-632X.12134

APA

Vancouver

Author

Van Meervenne, Eva ; De Weirdt, Rosemarie ; Van Coillie, Els ; Devlieghere, Frank ; Herman, Lieve ; Boon, Nico. / Biofilm models for the food industry : hot spots for plasmid transfer?. In: Pathogens and Disease. 2014 ; Vol. 70, Nr. 3. blz. 332-8.

Bibtex

@article{7f5214e431b348288b2293df370a46db,
title = "Biofilm models for the food industry: hot spots for plasmid transfer?",
abstract = "Biofilms represent a substantial problem in the food industry, with food spoilage, equipment failure, and public health aspects to consider. Besides, biofilms may be a hot spot for plasmid transfer, by which antibiotic resistance can be disseminated to potential foodborne pathogens. This study investigated biomass and plasmid transfer in dual-species (Pseudomonas putida and Escherichia coli) biofilm models relevant to the food industry. Two different configurations (flow-through and drip-flow) and two different inoculation procedures (donor-recipient and recipient-donor) were tested. The drip-flow configuration integrated stainless steel coupons in the setup while the flow-through configuration included a glass flow cell and silicone tubing. The highest biomass density [10 log (cells cm-²)] was obtained in the silicone tubing when first the recipient strain was inoculated. High plasmid transfer ratios, up to 1/10 (transconjugants/total bacteria), were found. Depending on the order of inoculation, a difference in transfer efficiency between the biofilm models could be found. The ease by which the multiresistance plasmid was transferred highlights the importance of biofilms in the food industry as hot spots for the acquisition of multiresistance plasmids. This can impede the treatment of foodborne illnesses if pathogens acquire this multiresistance in or from the biofilm.",
author = "{Van Meervenne}, Eva and {De Weirdt}, Rosemarie and {Van Coillie}, Els and Frank Devlieghere and Lieve Herman and Nico Boon",
note = "{\circledC} 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.",
year = "2014",
doi = "10.1111/2049-632X.12134",
language = "English",
volume = "70",
pages = "332--8",
journal = "Pathogens and Disease",
issn = "2049-632X",
publisher = "John Wiley & Sons",
number = "3",

}

RIS

TY - JOUR

T1 - Biofilm models for the food industry

T2 - hot spots for plasmid transfer?

AU - Van Meervenne, Eva

AU - De Weirdt, Rosemarie

AU - Van Coillie, Els

AU - Devlieghere, Frank

AU - Herman, Lieve

AU - Boon, Nico

N1 - © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.

PY - 2014

Y1 - 2014

N2 - Biofilms represent a substantial problem in the food industry, with food spoilage, equipment failure, and public health aspects to consider. Besides, biofilms may be a hot spot for plasmid transfer, by which antibiotic resistance can be disseminated to potential foodborne pathogens. This study investigated biomass and plasmid transfer in dual-species (Pseudomonas putida and Escherichia coli) biofilm models relevant to the food industry. Two different configurations (flow-through and drip-flow) and two different inoculation procedures (donor-recipient and recipient-donor) were tested. The drip-flow configuration integrated stainless steel coupons in the setup while the flow-through configuration included a glass flow cell and silicone tubing. The highest biomass density [10 log (cells cm-²)] was obtained in the silicone tubing when first the recipient strain was inoculated. High plasmid transfer ratios, up to 1/10 (transconjugants/total bacteria), were found. Depending on the order of inoculation, a difference in transfer efficiency between the biofilm models could be found. The ease by which the multiresistance plasmid was transferred highlights the importance of biofilms in the food industry as hot spots for the acquisition of multiresistance plasmids. This can impede the treatment of foodborne illnesses if pathogens acquire this multiresistance in or from the biofilm.

AB - Biofilms represent a substantial problem in the food industry, with food spoilage, equipment failure, and public health aspects to consider. Besides, biofilms may be a hot spot for plasmid transfer, by which antibiotic resistance can be disseminated to potential foodborne pathogens. This study investigated biomass and plasmid transfer in dual-species (Pseudomonas putida and Escherichia coli) biofilm models relevant to the food industry. Two different configurations (flow-through and drip-flow) and two different inoculation procedures (donor-recipient and recipient-donor) were tested. The drip-flow configuration integrated stainless steel coupons in the setup while the flow-through configuration included a glass flow cell and silicone tubing. The highest biomass density [10 log (cells cm-²)] was obtained in the silicone tubing when first the recipient strain was inoculated. High plasmid transfer ratios, up to 1/10 (transconjugants/total bacteria), were found. Depending on the order of inoculation, a difference in transfer efficiency between the biofilm models could be found. The ease by which the multiresistance plasmid was transferred highlights the importance of biofilms in the food industry as hot spots for the acquisition of multiresistance plasmids. This can impede the treatment of foodborne illnesses if pathogens acquire this multiresistance in or from the biofilm.

U2 - 10.1111/2049-632X.12134

DO - 10.1111/2049-632X.12134

M3 - A1: Web of Science-article

VL - 70

SP - 332

EP - 338

JO - Pathogens and Disease

JF - Pathogens and Disease

SN - 2049-632X

IS - 3

ER -