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Biological control of Rhizoctonia root rot on bean by phenazine- and cyclic lipopeptide-producing Pseudomonas CMR12a. / D'aes, Jolien; Hua, Gia Khuong Hoang; De Maeyer, Katrien; Pannecoucque, Joke; Forrez, Ilse; Ongena, Marc; Dietrich, Lars E. P.; Thomashow, Linda S.; Mavrodi, Dmitri V.; Hofte, Monica.

In: Phytopahology, Vol. 101, Nr. 8, 01.08.2011, blz. 996-1004.

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikelOnderzoekpeer review

Harvard

D'aes, J, Hua, GKH, De Maeyer, K, Pannecoucque, J, Forrez, I, Ongena, M, Dietrich, LEP, Thomashow, LS, Mavrodi, DV & Hofte, M 2011, 'Biological control of Rhizoctonia root rot on bean by phenazine- and cyclic lipopeptide-producing Pseudomonas CMR12a' Phytopahology, vol. 101, nr. 8, blz. 996-1004. https://doi.org/10.1094/PHYTO-11-10-0315

APA

D'aes, J., Hua, G. K. H., De Maeyer, K., Pannecoucque, J., Forrez, I., Ongena, M., ... Hofte, M. (2011). Biological control of Rhizoctonia root rot on bean by phenazine- and cyclic lipopeptide-producing Pseudomonas CMR12a. Phytopahology, 101(8), 996-1004. https://doi.org/10.1094/PHYTO-11-10-0315

Vancouver

Author

D'aes, Jolien ; Hua, Gia Khuong Hoang ; De Maeyer, Katrien ; Pannecoucque, Joke ; Forrez, Ilse ; Ongena, Marc ; Dietrich, Lars E. P. ; Thomashow, Linda S. ; Mavrodi, Dmitri V. ; Hofte, Monica. / Biological control of Rhizoctonia root rot on bean by phenazine- and cyclic lipopeptide-producing Pseudomonas CMR12a. In: Phytopahology. 2011 ; Vol. 101, Nr. 8. blz. 996-1004.

Bibtex

@article{2f8d916f3f3e424b802a505f5bc03d58,
title = "Biological control of Rhizoctonia root rot on bean by phenazine- and cyclic lipopeptide-producing Pseudomonas CMR12a",
abstract = "Pseudomonas CMR12a was previously selected as an efficient biocontrol strain producing phenazines and cyclic lipopeptides (CLPs). In this study, biocontrol capacity of Pseudomonas CMR12a against Rhizoctonia root rot of bean and the involvement of phenazines and CLPs in this ability were tested. Two different anastomosis groups (AGs) of Rhizoctonia solani, the intermediately aggressive AG 2-2 and the highly aggressive AG 4 HGI, were included in growth-chamber experiments with bean plants. The wild-type strain CMR12a dramatically reduced disease severity caused by both R. solani AGs. A CLP-deficient and a phenazine-deficient mutant of CMR12a still protected bean plants, albeit to a lesser extent compared with the wild type. Two mutants deficient in both phenazine and CLP production completely lost their biocontrol activity. Disease-suppressive capacity of CMR12a decreased after washing bacteria before application to soil and thereby removing metabolites produced during growth on plate. In addition, microscopic observations revealed pronounced branching of hyphal tips of both R. solani AGs in the presence of CMR12a. More branched and denser mycelium was also observed for the phenazine-deficient mutant; however, neither the CLP-deficient mutant nor the mutants deficient in both CLPs and phenazines influenced hyphal growth. Together, results demonstrate the involvement of phenazines and CLPs during Pseudomonas CMR12a-mediated biocontrol of Rhizoctonia root rot of bean.",
author = "Jolien D'aes and Hua, {Gia Khuong Hoang} and {De Maeyer}, Katrien and Joke Pannecoucque and Ilse Forrez and Marc Ongena and Dietrich, {Lars E. P.} and Thomashow, {Linda S.} and Mavrodi, {Dmitri V.} and Monica Hofte",
year = "2011",
month = "8",
day = "1",
doi = "10.1094/PHYTO-11-10-0315",
language = "English",
volume = "101",
pages = "996--1004",
journal = "Phytopathology",
issn = "0031-949X",
publisher = "AMER PHYTOPATHOLOGICAL SOC",
number = "8",

}

RIS

TY - JOUR

T1 - Biological control of Rhizoctonia root rot on bean by phenazine- and cyclic lipopeptide-producing Pseudomonas CMR12a

AU - D'aes, Jolien

AU - Hua, Gia Khuong Hoang

AU - De Maeyer, Katrien

AU - Pannecoucque, Joke

AU - Forrez, Ilse

AU - Ongena, Marc

AU - Dietrich, Lars E. P.

AU - Thomashow, Linda S.

AU - Mavrodi, Dmitri V.

AU - Hofte, Monica

PY - 2011/8/1

Y1 - 2011/8/1

N2 - Pseudomonas CMR12a was previously selected as an efficient biocontrol strain producing phenazines and cyclic lipopeptides (CLPs). In this study, biocontrol capacity of Pseudomonas CMR12a against Rhizoctonia root rot of bean and the involvement of phenazines and CLPs in this ability were tested. Two different anastomosis groups (AGs) of Rhizoctonia solani, the intermediately aggressive AG 2-2 and the highly aggressive AG 4 HGI, were included in growth-chamber experiments with bean plants. The wild-type strain CMR12a dramatically reduced disease severity caused by both R. solani AGs. A CLP-deficient and a phenazine-deficient mutant of CMR12a still protected bean plants, albeit to a lesser extent compared with the wild type. Two mutants deficient in both phenazine and CLP production completely lost their biocontrol activity. Disease-suppressive capacity of CMR12a decreased after washing bacteria before application to soil and thereby removing metabolites produced during growth on plate. In addition, microscopic observations revealed pronounced branching of hyphal tips of both R. solani AGs in the presence of CMR12a. More branched and denser mycelium was also observed for the phenazine-deficient mutant; however, neither the CLP-deficient mutant nor the mutants deficient in both CLPs and phenazines influenced hyphal growth. Together, results demonstrate the involvement of phenazines and CLPs during Pseudomonas CMR12a-mediated biocontrol of Rhizoctonia root rot of bean.

AB - Pseudomonas CMR12a was previously selected as an efficient biocontrol strain producing phenazines and cyclic lipopeptides (CLPs). In this study, biocontrol capacity of Pseudomonas CMR12a against Rhizoctonia root rot of bean and the involvement of phenazines and CLPs in this ability were tested. Two different anastomosis groups (AGs) of Rhizoctonia solani, the intermediately aggressive AG 2-2 and the highly aggressive AG 4 HGI, were included in growth-chamber experiments with bean plants. The wild-type strain CMR12a dramatically reduced disease severity caused by both R. solani AGs. A CLP-deficient and a phenazine-deficient mutant of CMR12a still protected bean plants, albeit to a lesser extent compared with the wild type. Two mutants deficient in both phenazine and CLP production completely lost their biocontrol activity. Disease-suppressive capacity of CMR12a decreased after washing bacteria before application to soil and thereby removing metabolites produced during growth on plate. In addition, microscopic observations revealed pronounced branching of hyphal tips of both R. solani AGs in the presence of CMR12a. More branched and denser mycelium was also observed for the phenazine-deficient mutant; however, neither the CLP-deficient mutant nor the mutants deficient in both CLPs and phenazines influenced hyphal growth. Together, results demonstrate the involvement of phenazines and CLPs during Pseudomonas CMR12a-mediated biocontrol of Rhizoctonia root rot of bean.

U2 - 10.1094/PHYTO-11-10-0315

DO - 10.1094/PHYTO-11-10-0315

M3 - A1: Web of Science-article

VL - 101

SP - 996

EP - 1004

JO - Phytopathology

JF - Phytopathology

SN - 0031-949X

IS - 8

ER -