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Chromosome landing at the Arabidopsis TORNAD01 locus using an AFLP-based strategy. / Cnops, Gerda; denBoer, B; Gerats, A; VanMontagu, M; VanLijsebettens, M.

In: Molecular General Genetics, Vol. 253, Nr. 1-2, 1996, blz. 32-41.

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

Harvard

Cnops, G, denBoer, B, Gerats, A, VanMontagu, M & VanLijsebettens, M 1996, 'Chromosome landing at the Arabidopsis TORNAD01 locus using an AFLP-based strategy', Molecular General Genetics, vol. 253, nr. 1-2, blz. 32-41.

APA

Cnops, G., denBoer, B., Gerats, A., VanMontagu, M., & VanLijsebettens, M. (1996). Chromosome landing at the Arabidopsis TORNAD01 locus using an AFLP-based strategy. Molecular General Genetics, 253(1-2), 32-41.

Vancouver

Cnops G, denBoer B, Gerats A, VanMontagu M, VanLijsebettens M. Chromosome landing at the Arabidopsis TORNAD01 locus using an AFLP-based strategy. Molecular General Genetics. 1996;253(1-2):32-41.

Author

Cnops, Gerda ; denBoer, B ; Gerats, A ; VanMontagu, M ; VanLijsebettens, M. / Chromosome landing at the Arabidopsis TORNAD01 locus using an AFLP-based strategy. In: Molecular General Genetics. 1996 ; Vol. 253, Nr. 1-2. blz. 32-41.

Bibtex

@article{14eedff2cf644bc48d04ebfd23dcdd7b,
title = "Chromosome landing at the Arabidopsis TORNAD01 locus using an AFLP-based strategy",
abstract = "The Arabidopsis tornadol (trn1) mutation causes severe dwarfism combined with twisted growth of all organs. We present a chromosome landing strategy, using amplified restriction fragment length polymorphism (AFLP) marker technology, for the isolation of the TRN1 gene. The recessive trn1 mutation was identified in a C24 transgenic line and is located 5 cM from a T-DNA insertion. We mapped the TRN1 locus to the bottom half of chromosome 5 relative to visible and restriction fragment length polymorphism (RFLP) markers. Recombinant classes within a 3-cM region around TRN1 were used to build a high-resolution map in this region, using the AFLP technique. Approximately 300 primer combinations have been used to test about 26000 fragments for polymorphisms, Seventeen of these AFLP markers were identified in the 3-cM region around TRN1. These markers were mapped within this region using individual recombinants. Four of these AFLP markers co-segregate with TRN1 whereas one maps at one recombinant below TRN1. We isolated and cloned three of these AFLP markers. These markers identified two yeast artificial chromosome (YAC) clones, containing the RFLP marker above and the AFLP marker below TRN1, demonstrating that these YACs span the TRN1 locus and that chromosome landing has been achieved, using an AFLP-based strategy.",
author = "Gerda Cnops and B denBoer and A Gerats and M VanMontagu and M VanLijsebettens",
year = "1996",
language = "English",
volume = "253",
pages = "32--41",
journal = "Molecular General Genetics",
issn = "0026-8925",
publisher = "Springer Verlag",
number = "1-2",

}

RIS

TY - JOUR

T1 - Chromosome landing at the Arabidopsis TORNAD01 locus using an AFLP-based strategy

AU - Cnops, Gerda

AU - denBoer, B

AU - Gerats, A

AU - VanMontagu, M

AU - VanLijsebettens, M

PY - 1996

Y1 - 1996

N2 - The Arabidopsis tornadol (trn1) mutation causes severe dwarfism combined with twisted growth of all organs. We present a chromosome landing strategy, using amplified restriction fragment length polymorphism (AFLP) marker technology, for the isolation of the TRN1 gene. The recessive trn1 mutation was identified in a C24 transgenic line and is located 5 cM from a T-DNA insertion. We mapped the TRN1 locus to the bottom half of chromosome 5 relative to visible and restriction fragment length polymorphism (RFLP) markers. Recombinant classes within a 3-cM region around TRN1 were used to build a high-resolution map in this region, using the AFLP technique. Approximately 300 primer combinations have been used to test about 26000 fragments for polymorphisms, Seventeen of these AFLP markers were identified in the 3-cM region around TRN1. These markers were mapped within this region using individual recombinants. Four of these AFLP markers co-segregate with TRN1 whereas one maps at one recombinant below TRN1. We isolated and cloned three of these AFLP markers. These markers identified two yeast artificial chromosome (YAC) clones, containing the RFLP marker above and the AFLP marker below TRN1, demonstrating that these YACs span the TRN1 locus and that chromosome landing has been achieved, using an AFLP-based strategy.

AB - The Arabidopsis tornadol (trn1) mutation causes severe dwarfism combined with twisted growth of all organs. We present a chromosome landing strategy, using amplified restriction fragment length polymorphism (AFLP) marker technology, for the isolation of the TRN1 gene. The recessive trn1 mutation was identified in a C24 transgenic line and is located 5 cM from a T-DNA insertion. We mapped the TRN1 locus to the bottom half of chromosome 5 relative to visible and restriction fragment length polymorphism (RFLP) markers. Recombinant classes within a 3-cM region around TRN1 were used to build a high-resolution map in this region, using the AFLP technique. Approximately 300 primer combinations have been used to test about 26000 fragments for polymorphisms, Seventeen of these AFLP markers were identified in the 3-cM region around TRN1. These markers were mapped within this region using individual recombinants. Four of these AFLP markers co-segregate with TRN1 whereas one maps at one recombinant below TRN1. We isolated and cloned three of these AFLP markers. These markers identified two yeast artificial chromosome (YAC) clones, containing the RFLP marker above and the AFLP marker below TRN1, demonstrating that these YACs span the TRN1 locus and that chromosome landing has been achieved, using an AFLP-based strategy.

M3 - A1: Web of Science-article

VL - 253

SP - 32

EP - 41

JO - Molecular General Genetics

JF - Molecular General Genetics

SN - 0026-8925

IS - 1-2

ER -