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Comparing two techniques for viscosity measurements in poultry feedstuffs : does it render similar conclusions? / Khadem, Alireza; Lourenço, Marta; Delezie, Evelyne; Maertens, Luc; Goderis, Anne; Mombaerts, Ronny; Janssens, Geert P J.

In: Poultry science, Vol. 97, Nr. 9, 01.09.2018, blz. 3138-3148.

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikelOnderzoekpeer review

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Khadem, Alireza ; Lourenço, Marta ; Delezie, Evelyne ; Maertens, Luc ; Goderis, Anne ; Mombaerts, Ronny ; Janssens, Geert P J. / Comparing two techniques for viscosity measurements in poultry feedstuffs : does it render similar conclusions?. In: Poultry science. 2018 ; Vol. 97, Nr. 9. blz. 3138-3148.

Bibtex

@article{9b28b8d1258b42f5845cbd578ddd0298,
title = "Comparing two techniques for viscosity measurements in poultry feedstuffs: does it render similar conclusions?",
abstract = "Viscosity of intestinal contents is known to affect digestion and absorption of nutrients. In most poultry studies, intestinal viscosity has been measured only after complete removal of solid particles by centrifugation. Centrifugation may however remove particles that contribute to viscosity, hence giving rise to an underestimation of viscosity. Two viscosity measurement techniques, one including a centrifugation step (Brookfield) and the other without (Haake), were compared in-vitro to assess whether both techniques result in similar conclusions regarding viscosity in feedstuffs. Two sets of feedstuff preparations were used. The first set was prepared with different combinations of milled feedstuffs in order to have a wide range of viscosity: 100{\%} corn, 25{\%} corn + 75{\%} wheat, 100{\%} wheat, 90{\%} wheat + 10{\%} rye, all mixed with distilled water. In the second set, barley was incubated with different beta-glucanases, and soybean and sunflower meal were incubated with different pectinases, again all mixed with distilled water. Viscosity was assessed using both techniques (Haake and Brookfield equipments) at six different time points. To evaluate the extent of agreement between the two methods, the Lin's concordance correlation coefficient (CCC) was assessed using the percentage of increase in viscosity within each method, based on pairwise feedstuffs comparison (first set), or relative to the feedstuff without enzyme (second set). The rate of the agreement between the two methods was substantial for the first set of feedstuffs (66{\%}) and for the barley diets incubated with beta-glucanases (69{\%}), whereas the CCC score for the soybean meal diets was very poor (2{\%}) and fair for the sunflower meal diets, incubated with pectinases (32{\%}). The lack of agreement for the latter can be explained by the limited variation in viscosity in these low-viscous mixtures. Although the two techniques are considerably different (e.g., with or without preceding particle removal), they seem to render similar conclusions when applied to poultry feedstuffs to identify distinct differences under the tested circumstances.",
keywords = "Animal Feed, Animal Husbandry, Animals, Centrifugation, Gastrointestinal Contents, Poultry, Viscosity, Comparative Study, Journal Article",
author = "Alireza Khadem and Marta Louren{\cc}o and Evelyne Delezie and Luc Maertens and Anne Goderis and Ronny Mombaerts and Janssens, {Geert P J}",
year = "2018",
month = "9",
day = "1",
doi = "10.3382/ps/pey190",
language = "English",
volume = "97",
pages = "3138--3148",
journal = "Poultry Science",
issn = "0032-5791",
publisher = "Poultry Science Association",
number = "9",

}

RIS

TY - JOUR

T1 - Comparing two techniques for viscosity measurements in poultry feedstuffs

T2 - does it render similar conclusions?

AU - Khadem, Alireza

AU - Lourenço, Marta

AU - Delezie, Evelyne

AU - Maertens, Luc

AU - Goderis, Anne

AU - Mombaerts, Ronny

AU - Janssens, Geert P J

PY - 2018/9/1

Y1 - 2018/9/1

N2 - Viscosity of intestinal contents is known to affect digestion and absorption of nutrients. In most poultry studies, intestinal viscosity has been measured only after complete removal of solid particles by centrifugation. Centrifugation may however remove particles that contribute to viscosity, hence giving rise to an underestimation of viscosity. Two viscosity measurement techniques, one including a centrifugation step (Brookfield) and the other without (Haake), were compared in-vitro to assess whether both techniques result in similar conclusions regarding viscosity in feedstuffs. Two sets of feedstuff preparations were used. The first set was prepared with different combinations of milled feedstuffs in order to have a wide range of viscosity: 100% corn, 25% corn + 75% wheat, 100% wheat, 90% wheat + 10% rye, all mixed with distilled water. In the second set, barley was incubated with different beta-glucanases, and soybean and sunflower meal were incubated with different pectinases, again all mixed with distilled water. Viscosity was assessed using both techniques (Haake and Brookfield equipments) at six different time points. To evaluate the extent of agreement between the two methods, the Lin's concordance correlation coefficient (CCC) was assessed using the percentage of increase in viscosity within each method, based on pairwise feedstuffs comparison (first set), or relative to the feedstuff without enzyme (second set). The rate of the agreement between the two methods was substantial for the first set of feedstuffs (66%) and for the barley diets incubated with beta-glucanases (69%), whereas the CCC score for the soybean meal diets was very poor (2%) and fair for the sunflower meal diets, incubated with pectinases (32%). The lack of agreement for the latter can be explained by the limited variation in viscosity in these low-viscous mixtures. Although the two techniques are considerably different (e.g., with or without preceding particle removal), they seem to render similar conclusions when applied to poultry feedstuffs to identify distinct differences under the tested circumstances.

AB - Viscosity of intestinal contents is known to affect digestion and absorption of nutrients. In most poultry studies, intestinal viscosity has been measured only after complete removal of solid particles by centrifugation. Centrifugation may however remove particles that contribute to viscosity, hence giving rise to an underestimation of viscosity. Two viscosity measurement techniques, one including a centrifugation step (Brookfield) and the other without (Haake), were compared in-vitro to assess whether both techniques result in similar conclusions regarding viscosity in feedstuffs. Two sets of feedstuff preparations were used. The first set was prepared with different combinations of milled feedstuffs in order to have a wide range of viscosity: 100% corn, 25% corn + 75% wheat, 100% wheat, 90% wheat + 10% rye, all mixed with distilled water. In the second set, barley was incubated with different beta-glucanases, and soybean and sunflower meal were incubated with different pectinases, again all mixed with distilled water. Viscosity was assessed using both techniques (Haake and Brookfield equipments) at six different time points. To evaluate the extent of agreement between the two methods, the Lin's concordance correlation coefficient (CCC) was assessed using the percentage of increase in viscosity within each method, based on pairwise feedstuffs comparison (first set), or relative to the feedstuff without enzyme (second set). The rate of the agreement between the two methods was substantial for the first set of feedstuffs (66%) and for the barley diets incubated with beta-glucanases (69%), whereas the CCC score for the soybean meal diets was very poor (2%) and fair for the sunflower meal diets, incubated with pectinases (32%). The lack of agreement for the latter can be explained by the limited variation in viscosity in these low-viscous mixtures. Although the two techniques are considerably different (e.g., with or without preceding particle removal), they seem to render similar conclusions when applied to poultry feedstuffs to identify distinct differences under the tested circumstances.

KW - Animal Feed

KW - Animal Husbandry

KW - Animals

KW - Centrifugation

KW - Gastrointestinal Contents

KW - Poultry

KW - Viscosity

KW - Comparative Study

KW - Journal Article

U2 - 10.3382/ps/pey190

DO - 10.3382/ps/pey190

M3 - A1: Web of Science-article

VL - 97

SP - 3138

EP - 3148

JO - Poultry Science

JF - Poultry Science

SN - 0032-5791

IS - 9

ER -