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Development of a quantitative method for the simultaneous analysis of the boar taint compounds androstenone, skatole and indole in porcine serum and plasma by means of ultra-high performance liquid chromatography coupled to high resolution mass spectrometry. / Wauters, Jella; Vanden Bussche, Julie; Verplanken, Kaat; Bekaert, Karen; Aluwé, Marijke; Van den Broeke, Alice; Cousse, Annelies; Buys, Nadine; Vanhaecke, Lynn.

In: FOOD CHEMISTRY, Vol. 187, 2015, blz. 120-129.

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikel

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@article{82f5213aac2d467aab1ef4d04d0bb8a4,
title = "Development of a quantitative method for the simultaneous analysis of the boar taint compounds androstenone, skatole and indole in porcine serum and plasma by means of ultra-high performance liquid chromatography coupled to high resolution mass spectrometry",
abstract = "Boar taint is an off-odour occurring while heating meat or fat from boars. A method detecting the three compounds (androstenone, skatole and indole) simultaneously in blood would offer substantial advantages since it would allow monitoring the impact of rearing strategies. Therefore, a UHPLC-HR-Orbitrap-MS analysis method is optimized and validated for the quantification of these compounds in plasma or serum. Sample pre-treatment involved an extraction with diethylether followed by a centrifugal filtration (30 kDa). Limits of detection and quantification varied between 0.5 and 1 μg L(-1) and 2 and 3 μg L(-1) for the three compounds, respectively. Besides, an excellent repeatability (RSD < 7.6{\%}), within-laboratory reproducibility (RSD<10.5{\%}), recovery (87-97{\%}) and linearity (R(2)>0.99) were recorded. Correlations between serum/plasma and fat levels of the boar taint compounds were positive for skatole (r(serum) = 0.39 and r(plasma) = 0.84) and androstenone (r(serum) = 0.73-0.78 and r(plasma) = 0.32-0.80).",
author = "Jella Wauters and {Vanden Bussche}, Julie and Kaat Verplanken and Karen Bekaert and Marijke Aluw{\'e} and {Van den Broeke}, Alice and Annelies Cousse and Nadine Buys and Lynn Vanhaecke",
year = "2015",
language = "English",
volume = "187",
pages = "120--129",
journal = "FOOD CHEMISTRY",
issn = "0308-8146",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Development of a quantitative method for the simultaneous analysis of the boar taint compounds androstenone, skatole and indole in porcine serum and plasma by means of ultra-high performance liquid chromatography coupled to high resolution mass spectrometry

AU - Wauters, Jella

AU - Vanden Bussche, Julie

AU - Verplanken, Kaat

AU - Bekaert, Karen

AU - Aluwé, Marijke

AU - Van den Broeke, Alice

AU - Cousse, Annelies

AU - Buys, Nadine

AU - Vanhaecke, Lynn

PY - 2015

Y1 - 2015

N2 - Boar taint is an off-odour occurring while heating meat or fat from boars. A method detecting the three compounds (androstenone, skatole and indole) simultaneously in blood would offer substantial advantages since it would allow monitoring the impact of rearing strategies. Therefore, a UHPLC-HR-Orbitrap-MS analysis method is optimized and validated for the quantification of these compounds in plasma or serum. Sample pre-treatment involved an extraction with diethylether followed by a centrifugal filtration (30 kDa). Limits of detection and quantification varied between 0.5 and 1 μg L(-1) and 2 and 3 μg L(-1) for the three compounds, respectively. Besides, an excellent repeatability (RSD < 7.6%), within-laboratory reproducibility (RSD<10.5%), recovery (87-97%) and linearity (R(2)>0.99) were recorded. Correlations between serum/plasma and fat levels of the boar taint compounds were positive for skatole (r(serum) = 0.39 and r(plasma) = 0.84) and androstenone (r(serum) = 0.73-0.78 and r(plasma) = 0.32-0.80).

AB - Boar taint is an off-odour occurring while heating meat or fat from boars. A method detecting the three compounds (androstenone, skatole and indole) simultaneously in blood would offer substantial advantages since it would allow monitoring the impact of rearing strategies. Therefore, a UHPLC-HR-Orbitrap-MS analysis method is optimized and validated for the quantification of these compounds in plasma or serum. Sample pre-treatment involved an extraction with diethylether followed by a centrifugal filtration (30 kDa). Limits of detection and quantification varied between 0.5 and 1 μg L(-1) and 2 and 3 μg L(-1) for the three compounds, respectively. Besides, an excellent repeatability (RSD < 7.6%), within-laboratory reproducibility (RSD<10.5%), recovery (87-97%) and linearity (R(2)>0.99) were recorded. Correlations between serum/plasma and fat levels of the boar taint compounds were positive for skatole (r(serum) = 0.39 and r(plasma) = 0.84) and androstenone (r(serum) = 0.73-0.78 and r(plasma) = 0.32-0.80).

M3 - A1: Web of Science-article

VL - 187

SP - 120

EP - 129

JO - FOOD CHEMISTRY

JF - FOOD CHEMISTRY

SN - 0308-8146

ER -