Standard

EFFECT OF FOOD-BORNE FUSARIUM MYCOTOXINS ON THE GUT MICROBIOME COMPOSITION IN BROILER CHICKENS AND IN PIGS. / Antonissen, Gunther; Van Immerseel, Filip; Michiels, A.; Eeckhaut, Venessa; Vermeulen, K.; Reisinger , N.; Baeyen, Steve; Haegeman, Annelies; Audenaert, K; De Saeger, Sarah; Maes, Dominiek; Haesebrouck, Freddy; Ducatelle, Richard; Croubels, Siska.

EFFECT OF FOOD-BORNE FUSARIUM MYCOTOXINS ON THE GUT MICROBIOME COMPOSITION IN BROILER CHICKENS AND IN PIGS . 2017.

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureC3: Congres abstract

Harvard

Antonissen, G, Van Immerseel, F, Michiels, A, Eeckhaut, V, Vermeulen, K, Reisinger , N, Baeyen, S, Haegeman, A, Audenaert, K, De Saeger, S, Maes, D, Haesebrouck, F, Ducatelle, R & Croubels, S 2017, EFFECT OF FOOD-BORNE FUSARIUM MYCOTOXINS ON THE GUT MICROBIOME COMPOSITION IN BROILER CHICKENS AND IN PIGS. in EFFECT OF FOOD-BORNE FUSARIUM MYCOTOXINS ON THE GUT MICROBIOME COMPOSITION IN BROILER CHICKENS AND IN PIGS . 1st MycoKey International Conference , Gent, België, 11/09/17.

APA

Antonissen, G., Van Immerseel, F., Michiels, A., Eeckhaut, V., Vermeulen, K., Reisinger , N., ... Croubels, S. (2017). EFFECT OF FOOD-BORNE FUSARIUM MYCOTOXINS ON THE GUT MICROBIOME COMPOSITION IN BROILER CHICKENS AND IN PIGS. In EFFECT OF FOOD-BORNE FUSARIUM MYCOTOXINS ON THE GUT MICROBIOME COMPOSITION IN BROILER CHICKENS AND IN PIGS

Vancouver

Antonissen G, Van Immerseel F, Michiels A, Eeckhaut V, Vermeulen K, Reisinger N et al. EFFECT OF FOOD-BORNE FUSARIUM MYCOTOXINS ON THE GUT MICROBIOME COMPOSITION IN BROILER CHICKENS AND IN PIGS. In EFFECT OF FOOD-BORNE FUSARIUM MYCOTOXINS ON THE GUT MICROBIOME COMPOSITION IN BROILER CHICKENS AND IN PIGS . 2017

Author

Antonissen, Gunther ; Van Immerseel, Filip ; Michiels, A. ; Eeckhaut, Venessa ; Vermeulen, K. ; Reisinger , N. ; Baeyen, Steve ; Haegeman, Annelies ; Audenaert, K ; De Saeger, Sarah ; Maes, Dominiek ; Haesebrouck, Freddy ; Ducatelle, Richard ; Croubels, Siska. / EFFECT OF FOOD-BORNE FUSARIUM MYCOTOXINS ON THE GUT MICROBIOME COMPOSITION IN BROILER CHICKENS AND IN PIGS. EFFECT OF FOOD-BORNE FUSARIUM MYCOTOXINS ON THE GUT MICROBIOME COMPOSITION IN BROILER CHICKENS AND IN PIGS . 2017.

Bibtex

@inbook{46f9ad22db7f4c7e820d8e71427e56b2,
title = "EFFECT OF FOOD-BORNE FUSARIUM MYCOTOXINS ON THE GUT MICROBIOME COMPOSITION IN BROILER CHICKENS AND IN PIGS",
abstract = "The mycotoxins deoxynivalenol (DON) and fumonisins (FBs) exert toxicity toward the intestinal epithelial barrier and immune system. Besides, following ingestion of contaminated feed, the intestinal microbiota will be exposed to these toxins. The gut harbours a complex community of over trillions microbial cells which influence animal physiology, metabolism, nutrition and immune function, while disruption of the gut microbiota has been linked with poor intestinal health. The aim of this presentation is to discuss comparatively the impact of mycotoxins on intestinal microbiota composition in broiler chickens and pigs. In a first study, the impact of DON and FBs on the composition of the intestinal microbiota in broiler chickens of different ages was assessed. Chickens of 1 day old were fed either a control diet, a DON contaminated diet (3.3-3.7 mg DON/kg feed) or a FBs contaminated diet (15.5-18.3 mg FB1+FB2/kg feed). Subsequently, after a feeding period of one, three and five weeks chickens were euthanized and ileal and caecal content samples were collected. In a second study, the impact of DON and its acetylated forms on the gut microbiome in piglets of 3 weeks old was investigated. Piglets were fed either a control diet or a DON contaminated diet (0.4 mg DON, 0.3 mg 3-acetylDON, and 0.8 mg 15-acetylDON/kg feed) for 5 weeks. Following euthanasia, colon content samples were collected. Intestinal microbiome profiling was performed by next-generation sequencing of 16S ribosomal DNA. The hyper-variable V4 region was amplified by using the 515F-806R primer set, and the amplicon library was prepared with Nextera adaptors. Subsequently, PCR fragments were sequenced by MiSeq Illumina sequencing (96 samples per lane, 2x300bp 5Gb raw data per lane). The results showed that the impact of DON and FBs on the intestinal microbiota is species- and age-dependent. Mycotoxin exposure modulated the abundance of several members of the family of Lachnospiraceae, Ruminococcacceae, Peptococaceae, Coriobacteriaceae, Bifidobacteriaceae, Clostridiaceae, and Veillonellaceae. Several of the affected microbial genera are involved in the production of short-chain fatty acids such as butyric acid.",
author = "Gunther Antonissen and {Van Immerseel}, Filip and A. Michiels and Venessa Eeckhaut and K. Vermeulen and N. Reisinger and Steve Baeyen and Annelies Haegeman and K Audenaert and {De Saeger}, Sarah and Dominiek Maes and Freddy Haesebrouck and Richard Ducatelle and Siska Croubels",
note = "abstract for the first MycoKey international Conferenceheld in Ghent, Belgium from 11 to 14 September 2017",
year = "2017",
month = "9",
day = "11",
language = "English",
booktitle = "EFFECT OF FOOD-BORNE FUSARIUM MYCOTOXINS ON THE GUT MICROBIOME COMPOSITION IN BROILER CHICKENS AND IN PIGS",

}

RIS

TY - CHAP

T1 - EFFECT OF FOOD-BORNE FUSARIUM MYCOTOXINS ON THE GUT MICROBIOME COMPOSITION IN BROILER CHICKENS AND IN PIGS

AU - Antonissen, Gunther

AU - Van Immerseel, Filip

AU - Michiels, A.

AU - Eeckhaut, Venessa

AU - Vermeulen, K.

AU - Reisinger , N.

AU - Baeyen, Steve

AU - Haegeman, Annelies

AU - Audenaert, K

AU - De Saeger, Sarah

AU - Maes, Dominiek

AU - Haesebrouck, Freddy

AU - Ducatelle, Richard

AU - Croubels, Siska

N1 - abstract for the first MycoKey international Conferenceheld in Ghent, Belgium from 11 to 14 September 2017

PY - 2017/9/11

Y1 - 2017/9/11

N2 - The mycotoxins deoxynivalenol (DON) and fumonisins (FBs) exert toxicity toward the intestinal epithelial barrier and immune system. Besides, following ingestion of contaminated feed, the intestinal microbiota will be exposed to these toxins. The gut harbours a complex community of over trillions microbial cells which influence animal physiology, metabolism, nutrition and immune function, while disruption of the gut microbiota has been linked with poor intestinal health. The aim of this presentation is to discuss comparatively the impact of mycotoxins on intestinal microbiota composition in broiler chickens and pigs. In a first study, the impact of DON and FBs on the composition of the intestinal microbiota in broiler chickens of different ages was assessed. Chickens of 1 day old were fed either a control diet, a DON contaminated diet (3.3-3.7 mg DON/kg feed) or a FBs contaminated diet (15.5-18.3 mg FB1+FB2/kg feed). Subsequently, after a feeding period of one, three and five weeks chickens were euthanized and ileal and caecal content samples were collected. In a second study, the impact of DON and its acetylated forms on the gut microbiome in piglets of 3 weeks old was investigated. Piglets were fed either a control diet or a DON contaminated diet (0.4 mg DON, 0.3 mg 3-acetylDON, and 0.8 mg 15-acetylDON/kg feed) for 5 weeks. Following euthanasia, colon content samples were collected. Intestinal microbiome profiling was performed by next-generation sequencing of 16S ribosomal DNA. The hyper-variable V4 region was amplified by using the 515F-806R primer set, and the amplicon library was prepared with Nextera adaptors. Subsequently, PCR fragments were sequenced by MiSeq Illumina sequencing (96 samples per lane, 2x300bp 5Gb raw data per lane). The results showed that the impact of DON and FBs on the intestinal microbiota is species- and age-dependent. Mycotoxin exposure modulated the abundance of several members of the family of Lachnospiraceae, Ruminococcacceae, Peptococaceae, Coriobacteriaceae, Bifidobacteriaceae, Clostridiaceae, and Veillonellaceae. Several of the affected microbial genera are involved in the production of short-chain fatty acids such as butyric acid.

AB - The mycotoxins deoxynivalenol (DON) and fumonisins (FBs) exert toxicity toward the intestinal epithelial barrier and immune system. Besides, following ingestion of contaminated feed, the intestinal microbiota will be exposed to these toxins. The gut harbours a complex community of over trillions microbial cells which influence animal physiology, metabolism, nutrition and immune function, while disruption of the gut microbiota has been linked with poor intestinal health. The aim of this presentation is to discuss comparatively the impact of mycotoxins on intestinal microbiota composition in broiler chickens and pigs. In a first study, the impact of DON and FBs on the composition of the intestinal microbiota in broiler chickens of different ages was assessed. Chickens of 1 day old were fed either a control diet, a DON contaminated diet (3.3-3.7 mg DON/kg feed) or a FBs contaminated diet (15.5-18.3 mg FB1+FB2/kg feed). Subsequently, after a feeding period of one, three and five weeks chickens were euthanized and ileal and caecal content samples were collected. In a second study, the impact of DON and its acetylated forms on the gut microbiome in piglets of 3 weeks old was investigated. Piglets were fed either a control diet or a DON contaminated diet (0.4 mg DON, 0.3 mg 3-acetylDON, and 0.8 mg 15-acetylDON/kg feed) for 5 weeks. Following euthanasia, colon content samples were collected. Intestinal microbiome profiling was performed by next-generation sequencing of 16S ribosomal DNA. The hyper-variable V4 region was amplified by using the 515F-806R primer set, and the amplicon library was prepared with Nextera adaptors. Subsequently, PCR fragments were sequenced by MiSeq Illumina sequencing (96 samples per lane, 2x300bp 5Gb raw data per lane). The results showed that the impact of DON and FBs on the intestinal microbiota is species- and age-dependent. Mycotoxin exposure modulated the abundance of several members of the family of Lachnospiraceae, Ruminococcacceae, Peptococaceae, Coriobacteriaceae, Bifidobacteriaceae, Clostridiaceae, and Veillonellaceae. Several of the affected microbial genera are involved in the production of short-chain fatty acids such as butyric acid.

M3 - C3: Conference Abstract

BT - EFFECT OF FOOD-BORNE FUSARIUM MYCOTOXINS ON THE GUT MICROBIOME COMPOSITION IN BROILER CHICKENS AND IN PIGS

ER -