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Evidence that the stress hormone cortisol regulates biofilm formation differently among Flavobacterium columnare isolates. / Declercq, Annelies Maria; Cai, Wenlong; Naranjo, Eber; Thongda, Wilawan; Eeckhaut, Venessa; Bauwens, Eva; Arias, Covadonga; De La Fuente, Leonardo; Beck, Benjamin H; Lange, Miles D; Peatman, Eric; Haesebrouck, Freddy; Aerts, Johan; Decostere, Annemie.

In: Veterinary Research, Vol. 50, Nr. 1, 11.04.2019, blz. 24.

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Harvard

Declercq, AM, Cai, W, Naranjo, E, Thongda, W, Eeckhaut, V, Bauwens, E, Arias, C, De La Fuente, L, Beck, BH, Lange, MD, Peatman, E, Haesebrouck, F, Aerts, J & Decostere, A 2019, 'Evidence that the stress hormone cortisol regulates biofilm formation differently among Flavobacterium columnare isolates' Veterinary Research, vol. 50, nr. 1, blz. 24. https://doi.org/10.1186/s13567-019-0641-3

APA

Declercq, A. M., Cai, W., Naranjo, E., Thongda, W., Eeckhaut, V., Bauwens, E., ... Decostere, A. (2019). Evidence that the stress hormone cortisol regulates biofilm formation differently among Flavobacterium columnare isolates. Veterinary Research, 50(1), 24. https://doi.org/10.1186/s13567-019-0641-3

Vancouver

Author

Declercq, Annelies Maria ; Cai, Wenlong ; Naranjo, Eber ; Thongda, Wilawan ; Eeckhaut, Venessa ; Bauwens, Eva ; Arias, Covadonga ; De La Fuente, Leonardo ; Beck, Benjamin H ; Lange, Miles D ; Peatman, Eric ; Haesebrouck, Freddy ; Aerts, Johan ; Decostere, Annemie. / Evidence that the stress hormone cortisol regulates biofilm formation differently among Flavobacterium columnare isolates. In: Veterinary Research. 2019 ; Vol. 50, Nr. 1. blz. 24.

Bibtex

@article{c06f0a8ddb2d4cd9b15a92a8a14a16ca,
title = "Evidence that the stress hormone cortisol regulates biofilm formation differently among Flavobacterium columnare isolates",
abstract = "The impact of cortisol on Flavobacterium columnare biofilm formation was explored. Firstly, the dynamics of biofilm formation by one highly (HV) and one low virulent (LV) F. columnare isolate with and without the stress hormone cortisol under microfluidic flow conditions was characterized. This to confirm that F. columnare cells could form biofilm under cortisol supplementation, and to compare the temporal and structural differences between different treatment groups. One trial revealed that in both isolates cell aggregates resembling biofilms occurred within 7-h post-inoculation. Consequently, cell clusters were sloughed away, followed by a rebuilding of bacterial cell aggregates, suggestive for a high spreading capacity. While the HV isolate revealed cell aggregates formed upstream at all time-points, for the LV isolate this was only seen upon cortisol supplementation. Secondly, the transcriptional effect of genes (gldK, gldL, gldM, gldN, sprA, sprE, sprT, and porV) belonging to the Type IX secretion system involved in gliding motility was investigated in planktonic and biofilm cells of a HV and LV isolate to which no, a low (LD) or high (HD) dose of cortisol was added. Significantly lower expression of gliding genes gldK, gldL, gldM and gldN, and of protein secretion regulator porV was seen in the LV isolate planktonic cells supplemented with a HD-cortisol. The LV isolate biofilm cells treated with the HD-cortisol showed a significant upregulation of sprT, encoding mobile surface adhesion important in bacterial colonization. This is the first evidence for the co-regulatory effect of cortisol on biofilm formation and F. columnare gliding gene expression.",
author = "Declercq, {Annelies Maria} and Wenlong Cai and Eber Naranjo and Wilawan Thongda and Venessa Eeckhaut and Eva Bauwens and Covadonga Arias and {De La Fuente}, Leonardo and Beck, {Benjamin H} and Lange, {Miles D} and Eric Peatman and Freddy Haesebrouck and Johan Aerts and Annemie Decostere",
year = "2019",
month = "4",
day = "11",
doi = "10.1186/s13567-019-0641-3",
language = "English",
volume = "50",
pages = "24",
journal = "Veterinary Research",
issn = "0928-4249",
publisher = "BioMed Central",
number = "1",

}

RIS

TY - JOUR

T1 - Evidence that the stress hormone cortisol regulates biofilm formation differently among Flavobacterium columnare isolates

AU - Declercq, Annelies Maria

AU - Cai, Wenlong

AU - Naranjo, Eber

AU - Thongda, Wilawan

AU - Eeckhaut, Venessa

AU - Bauwens, Eva

AU - Arias, Covadonga

AU - De La Fuente, Leonardo

AU - Beck, Benjamin H

AU - Lange, Miles D

AU - Peatman, Eric

AU - Haesebrouck, Freddy

AU - Aerts, Johan

AU - Decostere, Annemie

PY - 2019/4/11

Y1 - 2019/4/11

N2 - The impact of cortisol on Flavobacterium columnare biofilm formation was explored. Firstly, the dynamics of biofilm formation by one highly (HV) and one low virulent (LV) F. columnare isolate with and without the stress hormone cortisol under microfluidic flow conditions was characterized. This to confirm that F. columnare cells could form biofilm under cortisol supplementation, and to compare the temporal and structural differences between different treatment groups. One trial revealed that in both isolates cell aggregates resembling biofilms occurred within 7-h post-inoculation. Consequently, cell clusters were sloughed away, followed by a rebuilding of bacterial cell aggregates, suggestive for a high spreading capacity. While the HV isolate revealed cell aggregates formed upstream at all time-points, for the LV isolate this was only seen upon cortisol supplementation. Secondly, the transcriptional effect of genes (gldK, gldL, gldM, gldN, sprA, sprE, sprT, and porV) belonging to the Type IX secretion system involved in gliding motility was investigated in planktonic and biofilm cells of a HV and LV isolate to which no, a low (LD) or high (HD) dose of cortisol was added. Significantly lower expression of gliding genes gldK, gldL, gldM and gldN, and of protein secretion regulator porV was seen in the LV isolate planktonic cells supplemented with a HD-cortisol. The LV isolate biofilm cells treated with the HD-cortisol showed a significant upregulation of sprT, encoding mobile surface adhesion important in bacterial colonization. This is the first evidence for the co-regulatory effect of cortisol on biofilm formation and F. columnare gliding gene expression.

AB - The impact of cortisol on Flavobacterium columnare biofilm formation was explored. Firstly, the dynamics of biofilm formation by one highly (HV) and one low virulent (LV) F. columnare isolate with and without the stress hormone cortisol under microfluidic flow conditions was characterized. This to confirm that F. columnare cells could form biofilm under cortisol supplementation, and to compare the temporal and structural differences between different treatment groups. One trial revealed that in both isolates cell aggregates resembling biofilms occurred within 7-h post-inoculation. Consequently, cell clusters were sloughed away, followed by a rebuilding of bacterial cell aggregates, suggestive for a high spreading capacity. While the HV isolate revealed cell aggregates formed upstream at all time-points, for the LV isolate this was only seen upon cortisol supplementation. Secondly, the transcriptional effect of genes (gldK, gldL, gldM, gldN, sprA, sprE, sprT, and porV) belonging to the Type IX secretion system involved in gliding motility was investigated in planktonic and biofilm cells of a HV and LV isolate to which no, a low (LD) or high (HD) dose of cortisol was added. Significantly lower expression of gliding genes gldK, gldL, gldM and gldN, and of protein secretion regulator porV was seen in the LV isolate planktonic cells supplemented with a HD-cortisol. The LV isolate biofilm cells treated with the HD-cortisol showed a significant upregulation of sprT, encoding mobile surface adhesion important in bacterial colonization. This is the first evidence for the co-regulatory effect of cortisol on biofilm formation and F. columnare gliding gene expression.

U2 - 10.1186/s13567-019-0641-3

DO - 10.1186/s13567-019-0641-3

M3 - Article

VL - 50

SP - 24

JO - Veterinary Research

JF - Veterinary Research

SN - 0928-4249

IS - 1

ER -