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Chrysanthemum, Chrysanthemum indicum (Asteraceae) (2n=6x=54), is globally the second economically most important floricultural crop. A possible alternative to generate new genetic variation is the creation of somatic hybrids. Hereto protocols for protoplast culture need to be available. Today, several authors have reported partial or complete regeneration, though only for a very limited number of genotypes. We have tested four genotypes using a Murashige and Skoog-based medium. Using liquid culture, 17.6 microcalli were obtained per 105 protoplasts for cultivar ‘Yoko Ono’, whereas experiments with the other cultivars yielded no microcalli. Other protoplast sources and protoplast densities could not induce sustained protoplast division in liquid cultures for the other genotypes tested. Calli were readily obtained from ‘Yoko Ono’ microcalli on MS supplemented with 5 mg/L KIN and 1 mg/L NAA. Globular somatic embryos were induced during callus culture on MS supplemented with BA (2 mg/L) and NAA (0.5 mg/L), whereas through further subculturing on IAA-enriched medium (1-10 mg/L), roots were induced.
Originele taal-2Engels
TitelProceedings of the 24th International Eucarpia Symposium Section Ornamentals: Ornamental Breeding Worldwide
EditorsT. Orlikowska
Volume953
Publicatiedatum2012
Pagina's325-329
ISBN van geprinte versie978-90-66054-17-2
StatusGepubliceerd - 2012
Event24th International Eucarpia Symposium, Section Ornamentals: Ornamental Breeding Worldwide - Warschau, Polen
Duur: 2-sep-20125-sep-2012

ID: 501494