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Induction of organogenesis from Chrysanthemum indicum protoplasts. / Eeckhaut, Tom; Van Huylenbroeck, Johan.

Proceedings of the 24th International Eucarpia Symposium Section Ornamentals: Ornamental Breeding Worldwide . ed. / T. Orlikowska. Vol. 953 2012. blz. 325-329 (Acta Horticulturae; No. 953).

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Harvard

Eeckhaut, T & Van Huylenbroeck, J 2012, Induction of organogenesis from Chrysanthemum indicum protoplasts. in T Orlikowska (ed.), Proceedings of the 24th International Eucarpia Symposium Section Ornamentals: Ornamental Breeding Worldwide . vol. 953, Acta Horticulturae, nr. 953, blz. 325-329, 24th International Eucarpia Symposium, Section Ornamentals: Ornamental Breeding Worldwide, Warschau, Polen, 2/09/12.

APA

Eeckhaut, T., & Van Huylenbroeck, J. (2012). Induction of organogenesis from Chrysanthemum indicum protoplasts. In T. Orlikowska (editor), Proceedings of the 24th International Eucarpia Symposium Section Ornamentals: Ornamental Breeding Worldwide (Vol. 953, blz. 325-329). (Acta Horticulturae; No. 953).

Vancouver

Eeckhaut T, Van Huylenbroeck J. Induction of organogenesis from Chrysanthemum indicum protoplasts. In Orlikowska T, editor, Proceedings of the 24th International Eucarpia Symposium Section Ornamentals: Ornamental Breeding Worldwide . Vol. 953. 2012. blz. 325-329. (Acta Horticulturae; 953).

Author

Eeckhaut, Tom ; Van Huylenbroeck, Johan. / Induction of organogenesis from Chrysanthemum indicum protoplasts. Proceedings of the 24th International Eucarpia Symposium Section Ornamentals: Ornamental Breeding Worldwide . editor / T. Orlikowska. Vol. 953 2012. blz. 325-329 (Acta Horticulturae; 953).

Bibtex

@inbook{195434ed5a4342e793c1445dbd51d99e,
title = "Induction of organogenesis from Chrysanthemum indicum protoplasts",
abstract = "Chrysanthemum, Chrysanthemum indicum (Asteraceae) (2n=6x=54), is globally the second economically most important floricultural crop. A possible alternative to generate new genetic variation is the creation of somatic hybrids. Hereto protocols for protoplast culture need to be available. Today, several authors have reported partial or complete regeneration, though only for a very limited number of genotypes. We have tested four genotypes using a Murashige and Skoog-based medium. Using liquid culture, 17.6 microcalli were obtained per 105 protoplasts for cultivar ‘Yoko Ono’, whereas experiments with the other cultivars yielded no microcalli. Other protoplast sources and protoplast densities could not induce sustained protoplast division in liquid cultures for the other genotypes tested. Calli were readily obtained from ‘Yoko Ono’ microcalli on MS supplemented with 5 mg/L KIN and 1 mg/L NAA. Globular somatic embryos were induced during callus culture on MS supplemented with BA (2 mg/L) and NAA (0.5 mg/L), whereas through further subculturing on IAA-enriched medium (1-10 mg/L), roots were induced.",
author = "Tom Eeckhaut and {Van Huylenbroeck}, Johan",
year = "2012",
language = "English",
isbn = "978-90-66054-17-2",
volume = "953",
series = "Acta Horticulturae",
publisher = "ISHS",
number = "953",
pages = "325--329",
editor = "T. Orlikowska",
booktitle = "Proceedings of the 24th International Eucarpia Symposium Section Ornamentals: Ornamental Breeding Worldwide",

}

RIS

TY - CHAP

T1 - Induction of organogenesis from Chrysanthemum indicum protoplasts

AU - Eeckhaut, Tom

AU - Van Huylenbroeck, Johan

PY - 2012

Y1 - 2012

N2 - Chrysanthemum, Chrysanthemum indicum (Asteraceae) (2n=6x=54), is globally the second economically most important floricultural crop. A possible alternative to generate new genetic variation is the creation of somatic hybrids. Hereto protocols for protoplast culture need to be available. Today, several authors have reported partial or complete regeneration, though only for a very limited number of genotypes. We have tested four genotypes using a Murashige and Skoog-based medium. Using liquid culture, 17.6 microcalli were obtained per 105 protoplasts for cultivar ‘Yoko Ono’, whereas experiments with the other cultivars yielded no microcalli. Other protoplast sources and protoplast densities could not induce sustained protoplast division in liquid cultures for the other genotypes tested. Calli were readily obtained from ‘Yoko Ono’ microcalli on MS supplemented with 5 mg/L KIN and 1 mg/L NAA. Globular somatic embryos were induced during callus culture on MS supplemented with BA (2 mg/L) and NAA (0.5 mg/L), whereas through further subculturing on IAA-enriched medium (1-10 mg/L), roots were induced.

AB - Chrysanthemum, Chrysanthemum indicum (Asteraceae) (2n=6x=54), is globally the second economically most important floricultural crop. A possible alternative to generate new genetic variation is the creation of somatic hybrids. Hereto protocols for protoplast culture need to be available. Today, several authors have reported partial or complete regeneration, though only for a very limited number of genotypes. We have tested four genotypes using a Murashige and Skoog-based medium. Using liquid culture, 17.6 microcalli were obtained per 105 protoplasts for cultivar ‘Yoko Ono’, whereas experiments with the other cultivars yielded no microcalli. Other protoplast sources and protoplast densities could not induce sustained protoplast division in liquid cultures for the other genotypes tested. Calli were readily obtained from ‘Yoko Ono’ microcalli on MS supplemented with 5 mg/L KIN and 1 mg/L NAA. Globular somatic embryos were induced during callus culture on MS supplemented with BA (2 mg/L) and NAA (0.5 mg/L), whereas through further subculturing on IAA-enriched medium (1-10 mg/L), roots were induced.

M3 - C1: Articles in proceedings

SN - 978-90-66054-17-2

VL - 953

T3 - Acta Horticulturae

SP - 325

EP - 329

BT - Proceedings of the 24th International Eucarpia Symposium Section Ornamentals: Ornamental Breeding Worldwide

A2 - Orlikowska, T.

ER -