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New sensitive and fast detection of Little cherry virus 1 using loop-mediated isothermal amplification (RT-LAMP). / Tahzima, Rachid; Foucart, Yoika; Peusens, Gertie; Belien, Tim; Massart, Sebastien; De Jonghe, Kris.

In: Journal of virological methods, Vol. 265, 10.01.2019, blz. 91-98.

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikelOnderzoekpeer review

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Tahzima, Rachid ; Foucart, Yoika ; Peusens, Gertie ; Belien, Tim ; Massart, Sebastien ; De Jonghe, Kris. / New sensitive and fast detection of Little cherry virus 1 using loop-mediated isothermal amplification (RT-LAMP). In: Journal of virological methods. 2019 ; Vol. 265. blz. 91-98.

Bibtex

@article{28b15fa4359d408a988a3dcfbab8f352,
title = "New sensitive and fast detection of Little cherry virus 1 using loop-mediated isothermal amplification (RT-LAMP)",
abstract = "Little cherry virus 1 (LChV-1) belongs to the genus Velarivirus, family Closteroviridae, is an economically important pathogen affecting mainly cherry around the world emphasizing the impetus for its efficient and accurate on-site detection. This study describes the development of a reliable diagnostic protocol of LChV-1 based on a one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP). The protocol detects LChV-1 isolates in less than 10 min by fluorescence monitoring using a mobile detection device and is most optimal when performed at 67 °C. Sharp melting curves and unique melting temperatures (Tm) were obtained for the positive samples. Both the RT-LAMP and classical RT-PCR methods are capable of specifically detecting LChV-1 in infected leaf tissues. In addition, the RT-LAMP has remarkable advantages in comparison to RT-PCR. It is at least hundred fold more sensitive, significantly faster (allowing on-field leaf-to-result diagnostic) and efficient at minimal cost. In conclusion, this innovative RT-LAMP approach can contribute to the implementation of sustainable integrated management strategies for detection of LChV-1 in commercial orchards or for horticultural research stations. It is also suitable for decision support in phytosanitary epidemiological programs.",
author = "Rachid Tahzima and Yoika Foucart and Gertie Peusens and Tim Belien and Sebastien Massart and {De Jonghe}, Kris",
year = "2019",
month = "1",
day = "10",
doi = "https://doi.org/10.1016/j.jviromet.2018.12.019",
language = "English",
volume = "265",
pages = "91--98",
journal = "Journal of virological methods",
issn = "0166-0934",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - New sensitive and fast detection of Little cherry virus 1 using loop-mediated isothermal amplification (RT-LAMP)

AU - Tahzima, Rachid

AU - Foucart, Yoika

AU - Peusens, Gertie

AU - Belien, Tim

AU - Massart, Sebastien

AU - De Jonghe, Kris

PY - 2019/1/10

Y1 - 2019/1/10

N2 - Little cherry virus 1 (LChV-1) belongs to the genus Velarivirus, family Closteroviridae, is an economically important pathogen affecting mainly cherry around the world emphasizing the impetus for its efficient and accurate on-site detection. This study describes the development of a reliable diagnostic protocol of LChV-1 based on a one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP). The protocol detects LChV-1 isolates in less than 10 min by fluorescence monitoring using a mobile detection device and is most optimal when performed at 67 °C. Sharp melting curves and unique melting temperatures (Tm) were obtained for the positive samples. Both the RT-LAMP and classical RT-PCR methods are capable of specifically detecting LChV-1 in infected leaf tissues. In addition, the RT-LAMP has remarkable advantages in comparison to RT-PCR. It is at least hundred fold more sensitive, significantly faster (allowing on-field leaf-to-result diagnostic) and efficient at minimal cost. In conclusion, this innovative RT-LAMP approach can contribute to the implementation of sustainable integrated management strategies for detection of LChV-1 in commercial orchards or for horticultural research stations. It is also suitable for decision support in phytosanitary epidemiological programs.

AB - Little cherry virus 1 (LChV-1) belongs to the genus Velarivirus, family Closteroviridae, is an economically important pathogen affecting mainly cherry around the world emphasizing the impetus for its efficient and accurate on-site detection. This study describes the development of a reliable diagnostic protocol of LChV-1 based on a one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP). The protocol detects LChV-1 isolates in less than 10 min by fluorescence monitoring using a mobile detection device and is most optimal when performed at 67 °C. Sharp melting curves and unique melting temperatures (Tm) were obtained for the positive samples. Both the RT-LAMP and classical RT-PCR methods are capable of specifically detecting LChV-1 in infected leaf tissues. In addition, the RT-LAMP has remarkable advantages in comparison to RT-PCR. It is at least hundred fold more sensitive, significantly faster (allowing on-field leaf-to-result diagnostic) and efficient at minimal cost. In conclusion, this innovative RT-LAMP approach can contribute to the implementation of sustainable integrated management strategies for detection of LChV-1 in commercial orchards or for horticultural research stations. It is also suitable for decision support in phytosanitary epidemiological programs.

U2 - https://doi.org/10.1016/j.jviromet.2018.12.019

DO - https://doi.org/10.1016/j.jviromet.2018.12.019

M3 - A1: Web of Science-article

VL - 265

SP - 91

EP - 98

JO - Journal of virological methods

JF - Journal of virological methods

SN - 0166-0934

ER -