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The impact of high throughput sequencing on plant health diagnostics. / Adams, Ian; Fox, Adrian; Boonham, Neil; Massart, Sebastien; De Jonghe, Kris.

In: European Journal of Plant Pathology, Vol. 152, Nr. 4, 12.2018, blz. 909-919.

Onderzoeksoutput: Bijdrage aan tijdschriftA1: Web of Science-artikelOnderzoekpeer review

Harvard

Adams, I, Fox, A, Boonham, N, Massart, S & De Jonghe, K 2018, 'The impact of high throughput sequencing on plant health diagnostics' European Journal of Plant Pathology, vol. 152, nr. 4, blz. 909-919. https://doi.org/• DOI: 10.1007/s10658-018-1570-0

APA

Adams, I., Fox, A., Boonham, N., Massart, S., & De Jonghe, K. (2018). The impact of high throughput sequencing on plant health diagnostics. European Journal of Plant Pathology, 152(4), 909-919. https://doi.org/• DOI: 10.1007/s10658-018-1570-0

Vancouver

Adams I, Fox A, Boonham N, Massart S, De Jonghe K. The impact of high throughput sequencing on plant health diagnostics. European Journal of Plant Pathology. 2018 dec;152(4):909-919. https://doi.org/• DOI: 10.1007/s10658-018-1570-0

Author

Adams, Ian ; Fox, Adrian ; Boonham, Neil ; Massart, Sebastien ; De Jonghe, Kris. / The impact of high throughput sequencing on plant health diagnostics. In: European Journal of Plant Pathology. 2018 ; Vol. 152, Nr. 4. blz. 909-919.

Bibtex

@article{af102d9cfe06468a8cd4648f06f0a618,
title = "The impact of high throughput sequencing on plant health diagnostics",
abstract = "High throughput sequencing informed diagnosticsis revolutionising plant pathology. The applicationof this technology is most advanced in plant virology,where it is already becoming a front-line diagnostictool and it is envisaged that for other types of pathogenand pests this will be the case in the near future. However,there are implications to deploying this technologydue to a number of technical and scientific challenges.Firstly, interpretation of data and the assessment of planthealth risk against a limited baseline of existing knowledgeof the presence of pathogens in a given geographicregion. Secondly, evidence of causality and the separationof pathogenic from commensal organisms in thesequence data, thirdly, the tension between the generationof a rapid sequence result with the necessary butlaborious epidemiological characterisation in support ofplant health risk assessment. Finally, the validation andaccreditation of methods based on this rapidly evolvingtechnology. These in turn present challenges for planthealth policy and regulation. This review discusses thedevelopment of this technology, its application in planthealth diagnostics, and explores the implications ofapplying this technology in the plant health setting.",
author = "Ian Adams and Adrian Fox and Neil Boonham and Sebastien Massart and {De Jonghe}, Kris",
year = "2018",
month = "12",
doi = "• DOI: 10.1007/s10658-018-1570-0",
language = "English",
volume = "152",
pages = "909--919",
journal = "European Journal of Plant Pathology",
issn = "0929-1873",
publisher = "Springer Netherlands",
number = "4",

}

RIS

TY - JOUR

T1 - The impact of high throughput sequencing on plant health diagnostics

AU - Adams, Ian

AU - Fox, Adrian

AU - Boonham, Neil

AU - Massart, Sebastien

AU - De Jonghe, Kris

PY - 2018/12

Y1 - 2018/12

N2 - High throughput sequencing informed diagnosticsis revolutionising plant pathology. The applicationof this technology is most advanced in plant virology,where it is already becoming a front-line diagnostictool and it is envisaged that for other types of pathogenand pests this will be the case in the near future. However,there are implications to deploying this technologydue to a number of technical and scientific challenges.Firstly, interpretation of data and the assessment of planthealth risk against a limited baseline of existing knowledgeof the presence of pathogens in a given geographicregion. Secondly, evidence of causality and the separationof pathogenic from commensal organisms in thesequence data, thirdly, the tension between the generationof a rapid sequence result with the necessary butlaborious epidemiological characterisation in support ofplant health risk assessment. Finally, the validation andaccreditation of methods based on this rapidly evolvingtechnology. These in turn present challenges for planthealth policy and regulation. This review discusses thedevelopment of this technology, its application in planthealth diagnostics, and explores the implications ofapplying this technology in the plant health setting.

AB - High throughput sequencing informed diagnosticsis revolutionising plant pathology. The applicationof this technology is most advanced in plant virology,where it is already becoming a front-line diagnostictool and it is envisaged that for other types of pathogenand pests this will be the case in the near future. However,there are implications to deploying this technologydue to a number of technical and scientific challenges.Firstly, interpretation of data and the assessment of planthealth risk against a limited baseline of existing knowledgeof the presence of pathogens in a given geographicregion. Secondly, evidence of causality and the separationof pathogenic from commensal organisms in thesequence data, thirdly, the tension between the generationof a rapid sequence result with the necessary butlaborious epidemiological characterisation in support ofplant health risk assessment. Finally, the validation andaccreditation of methods based on this rapidly evolvingtechnology. These in turn present challenges for planthealth policy and regulation. This review discusses thedevelopment of this technology, its application in planthealth diagnostics, and explores the implications ofapplying this technology in the plant health setting.

U2 - • DOI: 10.1007/s10658-018-1570-0

DO - • DOI: 10.1007/s10658-018-1570-0

M3 - A1: Web of Science-article

VL - 152

SP - 909

EP - 919

JO - European Journal of Plant Pathology

T2 - European Journal of Plant Pathology

JF - European Journal of Plant Pathology

SN - 0929-1873

IS - 4

ER -