Standard

Use of cloned DNA fragments as reference materials for event specific quantification of genetically modified organisms (GMOs). / Taverniers, I; Van Bockstaele, E; De Loose, M.

In: Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen), Vol. 66, Nr. 3b, 2001, blz. 469-72.

Onderzoeksoutput: Bijdrage aan tijdschriftA3: Artikel in een nationaal tijdschrift met peer review, dat niet inbegrepen is in A1 of A2

Harvard

Taverniers, I, Van Bockstaele, E & De Loose, M 2001, 'Use of cloned DNA fragments as reference materials for event specific quantification of genetically modified organisms (GMOs)', Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen), vol. 66, nr. 3b, blz. 469-72.

APA

Taverniers, I., Van Bockstaele, E., & De Loose, M. (2001). Use of cloned DNA fragments as reference materials for event specific quantification of genetically modified organisms (GMOs). Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen), 66(3b), 469-72.

Vancouver

Taverniers I, Van Bockstaele E, De Loose M. Use of cloned DNA fragments as reference materials for event specific quantification of genetically modified organisms (GMOs). Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen). 2001;66(3b):469-72.

Author

Taverniers, I ; Van Bockstaele, E ; De Loose, M. / Use of cloned DNA fragments as reference materials for event specific quantification of genetically modified organisms (GMOs). In: Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen). 2001 ; Vol. 66, Nr. 3b. blz. 469-72.

Bibtex

@article{660f6aeeb4ac45fca974a9b040f61ad7,
title = "Use of cloned DNA fragments as reference materials for event specific quantification of genetically modified organisms (GMOs)",
abstract = "For the quantification of genetically modified organisms (GMOs) in foods and feeds, real-time PCR is currently the most widely applied technique. To obtain a {\%} of GMO, a GMO-specific target sequence is quantified relatively to a species-specific sequence. The correctness and reliability of the obtained quantitative results fully depend on the reference materials used as standards for setting up external calibration curves. We introduced a completely new type of standards for quantification of GMOs, based on cloned plasmid DNA solutions with well-known amounts of the sequences of interest, expressed as copy numbers. Moreover, the junction sequence between inserted DNA and plant DNA was used as 'unique identifier'. In this study, the model was applied for Roundup Ready soybean.",
keywords = "Calibration, Cloning, Molecular, DNA Fragmentation, Food, Genetically Modified, Organisms, Genetically Modified, Plasmids, Polymerase Chain Reaction, Species Specificity",
author = "I Taverniers and {Van Bockstaele}, E and {De Loose}, M",
year = "2001",
language = "English",
volume = "66",
pages = "469--72",
journal = "Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)",
issn = "1373-7503",
publisher = "University of Gent",
number = "3b",

}

RIS

TY - JOUR

T1 - Use of cloned DNA fragments as reference materials for event specific quantification of genetically modified organisms (GMOs)

AU - Taverniers, I

AU - Van Bockstaele, E

AU - De Loose, M

PY - 2001

Y1 - 2001

N2 - For the quantification of genetically modified organisms (GMOs) in foods and feeds, real-time PCR is currently the most widely applied technique. To obtain a % of GMO, a GMO-specific target sequence is quantified relatively to a species-specific sequence. The correctness and reliability of the obtained quantitative results fully depend on the reference materials used as standards for setting up external calibration curves. We introduced a completely new type of standards for quantification of GMOs, based on cloned plasmid DNA solutions with well-known amounts of the sequences of interest, expressed as copy numbers. Moreover, the junction sequence between inserted DNA and plant DNA was used as 'unique identifier'. In this study, the model was applied for Roundup Ready soybean.

AB - For the quantification of genetically modified organisms (GMOs) in foods and feeds, real-time PCR is currently the most widely applied technique. To obtain a % of GMO, a GMO-specific target sequence is quantified relatively to a species-specific sequence. The correctness and reliability of the obtained quantitative results fully depend on the reference materials used as standards for setting up external calibration curves. We introduced a completely new type of standards for quantification of GMOs, based on cloned plasmid DNA solutions with well-known amounts of the sequences of interest, expressed as copy numbers. Moreover, the junction sequence between inserted DNA and plant DNA was used as 'unique identifier'. In this study, the model was applied for Roundup Ready soybean.

KW - Calibration

KW - Cloning, Molecular

KW - DNA Fragmentation

KW - Food, Genetically Modified

KW - Organisms, Genetically Modified

KW - Plasmids

KW - Polymerase Chain Reaction

KW - Species Specificity

M3 - A3: National journal article with peer review, not included in A1 or A2

C2 - 15954640

VL - 66

SP - 469

EP - 472

JO - Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)

JF - Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)

SN - 1373-7503

IS - 3b

ER -